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首页> 外文期刊>Food and Bioproducts Processing. Transactions of the Institution of Chemical Engineers, Part C >Evaluation of factors influencing dairy biofilm formation in filling hoses of food-processing equipment
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Evaluation of factors influencing dairy biofilm formation in filling hoses of food-processing equipment

机译：影响食品加工设备灌装软管中乳制品生物膜形成的因素评价

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Biofilms in dairy-processing environments lead to increased opportunities for microbial contamination of processed products. The aim of this work is to investigate the influence of factors that can affect biofilm onset in a dairy-filling test hose. Bacterial composition (monoculture Pseudomonas fragi, polyculture), fat content of milk (1.5%, 3.5%), flow condition (laminar, turbulent), and contact material (PTFE, stainless steel 1.4404) were considered as factors at a constant low temperature of 5.5 +/- 0.5 degrees C. Biofilms were visualized by microscopy (CLSM) and analysed by counting viable cells, ATP-bioluminescence assay, and staining method for biomass quantification. The correlation between these methods was evaluated, since the chosen methods detect different characteristics of biofilms. Furthermore, enzymatic cleaning was applied after each experimental run. The results showed that the bacterial composition, material type, and fat content significantly affect the results of viable-cell counting (p &= 0.05). Flow conditions and material type both have significant influence upon biomass-quantification results (p &= 0.001). The highest positive Pearson correlation was estimated between the ATP-bioluminescence and biomass methods (0.550), while the lowest correlation was found between viable-cell counting and biomass quantification (0.345). Enzymatic treatment showed a good result for the cleaning of the PTFE-hose liner in the dairy-filling hose. (C) 2018 Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.
机译：乳制品加工环境中的生物膜导致加工产品微生物污染的机会增加。这项工作的目的是探讨可能影响乳制品填充试验软管中生物膜发作的因素的影响。细菌组合物（单殖民伪霉菌碎片，多培养物），牛奶脂肪含量（1.5％，3.5％），流量（层状，湍流）和接触材料（PTFE，不锈钢1.4404）被认为是恒定低温的因素通过显微镜（CLSM）可视化5.5 +/- 0.5℃。通过计算活细胞，ATP-生物发光测定和用于生物质定量的染色方法来观察Biofilms。评估这些方法之间的相关性，因为所选择的方法检测生物膜的不同特征。此外，在每个实验过程中施加酶清洁。结果表明，细菌组合物，材料型和脂肪含量显着影响活性细胞计数的结果（P＆amp; = 0.05）。流动条件和材料类型对生物质定量结果有显着影响（P＆amp; = 0.001）。在ATP-生物发光和生物质方法（0.550）之间估计了最高的正皮子相关，而活性细胞计数和生物质定量（0.345）之间存在最低相关性的相关性。酶促处理表明，在乳制品填充软管中清洁PTFE-软管衬垫的良好结果。 （c）2018化学工程师机构。 elsevier b.v出版。保留所有权利。

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