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首页> 外文期刊>Gynecological endocrinology: the official journal of the International Society of Gynecological Endocrinology >Vitrified Wharton's jelly tissue as a biomaterial for multiple tissue engineering applications
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Vitrified Wharton's jelly tissue as a biomaterial for multiple tissue engineering applications

机译：玻璃化沃顿果冻组织作为多种组织工程应用的生物材料

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摘要

Wharton's Jelly (WJ) tissue is a promising biomaterial, for tissue engineering applications. However, its preservation over a long period in order to be readily available needs further optimization. A possible solution could be the vitrification and storage of WJ tissue at low temperatures. The aim of this study is to evaluate the effect of low temperature in the WJ tissue, which was stored at -196 degrees C, either with the vitrification or conventional cryopreservation methods. WJ tissues were isolated from human umbilical cords, cryopreserved with the above methods and remained for 1 year at -196 degrees C. Histological analysis of tissue's extracellular matrix (ECM), isolation, and characterization of mesenchymal stromal cells (MSCs) were performed. Histological analysis revealed the presence of ECM components such as collagen, sulfated glycosaminoglycans (sGAGs), and the presence of cell nuclei only in vitrified samples. Furthermore, MSCs were isolated and expanded successfully from vitrified WJ tissues, whereas a few viable cells were obtained from conventionally cryopreserved tissues that were not further expanded. In conclusion, this study indicated the proper preservation of vitrified WJ tissues after 1 year of storage, which eventually could be used in tissue engineering and regenerative medicine approaches.
机译：沃顿果冻（WJ）组织是一种有前途的生物材料，用于组织工程应用。然而，它的保存在很长一段时间内，以便易于获得需求进一步优化。可能的解决方案可以是低温下WJ组织的玻璃化和储存。本研究的目的是评估低温在WJ组织中的效果，其在-196℃下储存在玻璃化或常规的冷冻保存方法。从人脐带中分离出WJ组织，用上述方法冷冻保存并在-196摄氏度下保持1年。进行组织细胞外基质（ECM），分离和表征间充质基质细胞（MSCs）的组织学分析。组织学分析显示ECM组分如胶原蛋白，硫酸化糖胺聚糖（SGAG），以及仅在玻璃化样品中的细胞核存在。此外，将MSC分离并成功地从玻璃化WJ组织中膨胀，而从通常不进一步扩增的常规冷冻保存组织中获得几种活性细胞。总之，本研究表明，在1年内储存后适当保存玻璃化WJ组织，最终可用于组织工程和再生医学方法。

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