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首页> 外文期刊>Gynecological endocrinology: the official journal of the International Society of Gynecological Endocrinology >LC-MS/MS improves screening towards 21-hydroxylase deficiency
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LC-MS/MS improves screening towards 21-hydroxylase deficiency

机译：LC-MS / MS改善了21-羟化酶缺乏的筛选

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Basal serum 17OHP measurement remains the first screening step for nonclassic congenital adrenal hyperplasia (NCCAH) and the accuracy of the test is of high value. The aim of this study was to compare the accuracy of immunoassays to LC-MS/MS in the assessment of serum 17OHP and androgens concentration in women with hyperandrogenism and controls. 17OHP, total testosterone, androstendione and DHEA-S were measured in 39 women with clinically and/or biochemically evident hyperandrogenism and in 29 age-matched controls without clinical hyperandrogenism. 17OHP and androgens were measured by immunoassays and by LC-MS/MS. In patients group median 17OHP level measured by immunoassays was significantly higher compared to LC-MS/MS (5.49 nmol/l-ELISA NovaTec? and 3.57 nmol/l-ELISA DRG? versus 1.56nmol/l-LC-MS/MS p<0.0001) as well as in the control group (2.58nmol/ I-ELISA DRG? versus 1.14nmol/l-LC-MS/MS p< 0.0001). Additional, unnecessary diagnostic procedures explaining elevated 17OHP level were undertaken in 85% of patients when NovaTec? test was used, in 50% when ELISA DRG? and in none when LC-MS/MS method was applied. Total testosterone, androstendione and DHEA-S concentrations in the patients and the controls assessed by the immunoassays were also significantly higher compared to LC-MS/MS. LC-MS/MS is more reliable diagnostic tool in the measurement of serum 17OHP and androgens concentrations compared to immunoassays in women with hyperandrogenism.
机译：基础血清17OHP测量仍然是非染色先天性增生（NCCAH）的第一种筛选步骤，测试的准确性具有高价值。本研究的目的是将免疫测定与LC-MS / MS的准确性进行比较，以评估血清17Ohp和雌激素的雌激素和对照组的雌激素浓度。 17Ohp，总睾酮，androstendione和dhea-s在39名妇女中测量，临床和/或生物化学上明显的高衰变和29例，没有临床高糖母性的对照。通过免疫测定和通过LC-MS / MS测量17OHP和雄激素。在患者中，与LC-MS / MS相比，通过免疫测定测量的17 OHP水平显着提高（5.49酚/ L-ELISA NOVATEC？和3.57 Nmol / L-ELISA DRG？与1.56nmol / L-LC-MS / MS P < 0.0001）以及对照组（2.58nmol / I-ELISA DRGα，与1.14nmol / L-LC-MS / MS P <0.0001）。在NovaTec的85％的患者中，在85％的患者中进行了额外的不必要的诊断程序，在85％的患者中进行了呢？使用测试，在ELISA DRG时50％？当应用LC-MS / MS方法时，无论如何。与LC-MS / MS相比，患者中患者中的总睾酮，Androstendione和DHEA-S浓度以及免疫测定评估的对照也显着提高。 LC-MS / MS在血清17Ohp和雄甾醇浓度的测量中是更可靠的诊断工具，与患有高衰老症的女性的免疫测定相比。

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