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首页> 外文期刊>Gynecological endocrinology: the official journal of the International Society of Gynecological Endocrinology >Prostaglandin E-2 promotes BeWo spheroids implantation in RL95-2 cell monolayers
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Prostaglandin E-2 promotes BeWo spheroids implantation in RL95-2 cell monolayers

机译：前列腺素E-2在RL95-2细胞单层中促进Bewo球形植入

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Purpose: Integrin v3 (ITG v3) participates in the process of implantation between the embryo and the endometrium. This study investigated the effects of prostaglandin E-2 (PGE(2)) on endometrial receptivity and implantation efficiency of the embryo, and their possible mechanisms.Methods: Quantitative real-time reverse transcription PCR (qRT-PCR) and western blotting were used to detect the changes in mRNA and protein levels of ITG v3 in RL95-2 cells after administering PGE(2). BeWo trophoblast cells and RL95-2 endometrial epithelial cells were used to establish an in vitro model, which was used to observe the adhesion rate and spreading efficiency between BeWo spheroids and RL95-2 cell monolayers after pretreatment with different concentrations of PGE(2).Results: PGE(2) at 200nM increased the mRNA and protein levels of ITG v significantly (p<0.05); 100nM PGE(2) increased the mRNA and protein levels of ITG 3 significantly (p<0.05). PGE(2) at 200nM increased significantly the adhesion and spreading efficiency of BeWo spheres to RL95-2 cell monolayers.Conclusions: An appropriate concentration of PGE(2) might increase the expression of ITG v3, which would, promote embryo adhesion and spreading efficiency. This study provides further evidence that increased expression of ITG v3 might promote implantation by improving endometrial receptivity.
机译：目的：整合素V3（ITG V3）参与胚胎和子宫内膜之间的植入过程。本研究研究了前列腺素E-2（PGE（2））对胚胎的子宫内膜接受和植入效率的影响，以及它们可能的机制。方法：使用定量实时逆转录PCR（QRT-PCR）和蛋白质印迹在施用PGE（2）后检测R195-2细胞中ITG V3 mRNA和蛋白质水平的变化。 Bewo滋养细胞和R195-2子宫内膜上皮细胞用于建立体外模型，其用于观察在用不同浓度的PGE（2）的预处理后Bewo球状体和R195-2细胞单层之间的粘合速率和扩散效率。结果：PGE（2）在200nm时增加ITG V的mRNA和蛋白质水平显着（P <0.05）; 100nm PGE（2）显着增加ITG 3的mRNA和蛋白质水平（P <0.05）。 PGE（2）在200nm时显着增加Bewo球体对R195-2细胞单层的粘附和扩散效率。结论：适当浓度的PGE（2）可能会增加ITG V3的表达，这将促进胚胎粘附和扩散效率。本研究提供了进一步的证据，即通过改善子宫内膜接受性，ITG V3的表达增加可能促进植入。

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