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Dietary nucleotides can directly stimulate the immunity of zebrafish independent of the intestinal microbiota

机译：膳食核苷酸可以直接刺激斑马鱼的免疫，与肠道微生物群无关

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摘要

In this study, we firstly tested the effects of dietary nucleotides on the disease resistance and innate immunity of zebrafish. Further, we investigated the role of intestinal microbiota in the nucleotides-induced immunostimulatory effect by using a germ-free zebrafish model and microbiota transfer technique. Fish were fed control or nucleotides (NT)-supplemented diets (at 0.05%,0.1%, 0.15% or 0.2%, m/m) for 4 weeks, followed by immersion challenge with Aeromonas hydrophila NJ-1. The results showed that 0.1% NT group enhanced the resistance of zebrafish against A. hydrophila infection. We further observed that the relative expressions of mucin, claudin16, occlusin1, hepcidin, defensin beta-like, myeloperoxidase (Mpo), and serum amyloid A (Saa) increased in the 0.1% NT group compared with control (P & 0.05), indicating that dietary nucleotides enhanced the physical barrier and mucosal immunity in the intestine of zebrafish. Moreover, ROS level in the head kidney was significantly increased in NT fed zebrafish versus control (P & 0.05), indicating enhanced systematic immunity. Furthermore, dietary NT significantly elevated the relative expressions of mpo, saa and the ROS activity in germ-free zebrafish, while germ-free zebrafish colonized with NT-altered microbiota had no significant difference in the relative expressions of mpo, saa and the ROS activity compared with the control microbiotacolonized fish, suggesting that the immunostimulatory effect of dietary NT is mediated by direct action of NT and does not involve the microbiota. Consistently, dietary NT can protect germ-free zebrafish from pathogenic infection, whereas germ-free zebrafish colonized with NT microbiota showed no difference in disease resistance compared with control microbiota colonized counterparts. Together, these results indicated that the immunostimulatory and disease protection effect of dietary nucleotides in zebrafish was mediated by direct action of the nucleotides, and does not involve the intestinal microbiota.
机译：在这项研究中，我们首先测试了膳食核苷酸对斑马鱼抗病和先天免疫的影响。此外，我们通过使用无菌斑马鱼模型和微生物群转移技术来研究肠道微生物遗起在核苷酸诱导的免疫刺激作用中的作用。将鱼类喂养控制或核苷酸（NT） - 瓶种饮食（0.05％，0.1％，0.15％或0.2％，m / m），然后用Aeromonas疏水液NJ-1浸没攻击。结果表明，0.1％的NT组增强了斑马鱼对A.疏水菌感染的抗性。我们进一步观察到，与对照相比，0.1％NT组（P＆amp; 0.05 ），表明膳食核苷酸在斑马鱼的肠道中增强了物理屏障和粘膜免疫。此外，在NT喂养斑马鱼与对照（P＆amp; 0.05）中，头肾的ROS水平显着增加，表明增强了系统免疫力。此外，膳食NT显着升高了MPO，SAA和ROS活性在无菌斑马鱼中的相对表达，而用NT改变的微生物生物殖民殖民殖民在MPO，SAA和ROS活性的相对表达中没有显着差异与对照微生物凝固的鱼相比，表明膳食NT的免疫刺激作用是通过NT的直接作用介导的，并且不涉及微生物酵母。始终如一地，膳食NT可以通过致病感染来保护无菌斑马鱼，而用NT微生物群殖民殖民殖民殖民，与对照微生物群殖民化对应相比没有抗病性差异。这些结果表明，通过核苷酸的直接作用介导斑马鱼中膳食核苷酸的免疫刺激和疾病保护作用，并不涉及肠道微生物。

著录项

  • 来源
    《Fish & Shellfish Immunology》 |2019年第2019期|共8页
  • 作者

    Guo Xiaoze; Li Jie; Ran Chao; Wang Anran; Xie Mingxu; Xie Yadong; Ding Qianwen; Zhang Zhen; Yang Yalin; Duan Ming; Zhou Zhigang;

  • 作者单位

    Chinese Acad Agr Sci Feed Res Inst Sino Norway Joint Lab Fish Gut Microbiota Beijing Peoples R China;

    Chinese Acad Agr Sci Feed Res Inst Sino Norway Joint Lab Fish Gut Microbiota Beijing Peoples R China;

    Chinese Acad Agr Sci Feed Res Inst Sino Norway Joint Lab Fish Gut Microbiota Beijing Peoples R China;

    Chinese Acad Agr Sci Feed Res Inst Sino Norway Joint Lab Fish Gut Microbiota Beijing Peoples R China;

    Chinese Acad Agr Sci Feed Res Inst Sino Norway Joint Lab Fish Gut Microbiota Beijing Peoples R China;

    Chinese Acad Agr Sci Feed Res Inst Sino Norway Joint Lab Fish Gut Microbiota Beijing Peoples R China;

    Chinese Acad Agr Sci Feed Res Inst Sino Norway Joint Lab Fish Gut Microbiota Beijing Peoples R China;

    Chinese Acad Agr Sci Feed Res Inst Sino Norway Joint Lab Fish Gut Microbiota Beijing Peoples R China;

    Chinese Acad Agr Sci Feed Res Inst Sino Norway Joint Lab Fish Gut Microbiota Beijing Peoples R China;

    Chinese Acad Sci Inst Hydrobiol Stair Key Lab Freshwater Ecol &

    Biotechnol Wuhan 430072 Hubei Peoples R China;

    Chinese Acad Agr Sci Feed Res Inst Sino Norway Joint Lab Fish Gut Microbiota Beijing Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 水产、渔业;
  • 关键词

    Nucleotides; Intestinal microbiota; Innate immune response; Zebrafish;

    机译：核苷酸;肠道微生物群;天生的免疫反应;斑马鱼;

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