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首页> 外文期刊>Fish & Shellfish Immunology >Effects of dietary Bacillus subtilis and Shewanella algae in expression profile of immune-related genes from hemolymph of Litopenaeus vannamei challenged with Vibrio parahaemolyticus
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Effects of dietary Bacillus subtilis and Shewanella algae in expression profile of immune-related genes from hemolymph of Litopenaeus vannamei challenged with Vibrio parahaemolyticus

机译：膳食枯草芽孢杆菌和肺藻藻对Litopenaeus Vannamei血淋巴血小肿表达谱的影响抗血小肿血小肿抗原伞菌溶解

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摘要

B. subtilis and S. algae effects in growth, survival and innate immunity were assessed on L. vannamei juveniles. During 60 days, shrimp were reared in three treatments: Bs, fed with 10(6) CFU of B. subtilis per gram of commercial feed, Sa, fed with 10(6) CFU of S. algae per gram of commercial feed and Control (without bacterial addition). Then, the animals were subjected to a V. parahaemolyticus challenge. For this purpose, four treatments were established: Control (shrimp not submitted to probiotic treatments), Vibrio (Vibrio challenged shrimp), Vibrio + Bs (Bs challenged shrimp) and Vibrio + Sa (Sa challenged shrimp). Shrimp hemolymph was sampled 45-days after rearing and 24 h post-challenge for quantification of prophenoloxidase (proPO), lipopolysaccharide and beta-1,3-glucan-binding protein (LGBP) and hemocyanin (HEM) transcripts by qPCR. Moreover, shrimp final weight and survival were also verified. B. subtilis administration enhanced shrimp growth and improved proPO, LGBP and HEM expression levels before and after challenge. After 60-days of feeding, Sa final weight was higher than the Control, whereas Vibrio + Sa cumulative mortality after 48 h of Vibrio challenge was lower than Vibrio group. These results could be correlated with the proPO and LGBP up regulation in Vibrio + Sa compared to Vibrio group, protecting L. vannamei from the bacterial infection. Together, these results suggest the probiotic potential of B. subtilis e S. algae in the modulation of immune-related genes as a tool to control V. parahaemolyticus infection inside shrimp.
机译：B.在L.Vannamei青少年上评估了枯草芽孢杆菌和S.藻类生长，存活和先天免疫的影响。在60天内，虾饲养三种治疗方法：BS，用10（6）克B.枯草芽孢杆菌，每克商用饲料，SA喂养，喂食10（6）CFU每克商业饲料和控制（没有细菌加法）。然后，对动物进行V.副溶血性攻击。为此目的，建立了四种治疗方法：对照（虾类未提交给益生菌治疗），Vibrio（挑战虾），vibrio + bs（bs挑战虾）和vibrio + sa（sa挑战虾）。饲养后45天的虾血蜂氨酸在培训后45天，用于定量QPCR定量丙烯氧化酶（PROPO），脂多糖和β-1,3-葡聚糖结合蛋白（LGBP）和血红素素（HEM）转录物的挑战。此外，还验证了虾最终的重量和生存率。 B.枯草芽孢杆菌给药增强虾生长和改善的PROPO，LGBP和挑战后的下摆表达水平。喂养60天后，SA最终体重高于对照，而vibrio + sa累积死亡率在48小时后捕捞后低于vibrio组。与弧菌组相比，这些结果与vibro + sa的ProPo和LGBP调节相关，保护L. Vannamei免受细菌感染。这些结果表明B.枯草芽孢杆菌E S.藻类的益生菌潜力在调节免疫相关基因中作为控制虾中的V.副溶血性感染的工具。

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