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首页> 外文期刊>Fish & Shellfish Immunology >Novel Ca2+-independent C-type lectin involved in immune defense of the razor clam Sinonovacula constricta
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Novel Ca2+-independent C-type lectin involved in immune defense of the razor clam Sinonovacula constricta

机译：新型Ca2 + -Independent C型凝集凝集素，参与了剃刀蛤蜊Sinonovacula Crantricta的免疫防御

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摘要

C-type lectins (CTLs) are important pattern recognition molecules that participate in bacterial binding and agglutination by specific recognition of carbohydrates from pathogens. In this study, a full-length cDNA of CTL was cloned from Sinonovacula constricta (designated ScCTL-2). ScCTL-2 has a length of 981 bp, a 5'-untranslated region (UTR) of 47 bp, a short 3'-UTR of 37 bp, and an open reading frame (ORF) of 894 bp, which encodes a polypeptide of 298 amino acid residues. The deduced amino acid of ScCTL-2 possesses a conserved carbohydrate-recognition domain (CRD) similar to that of C-31-E-171. Spatial distribution analysis demonstrated that ScCTL-2 was constitutively expressed in all tested tissues, with dominant expression in foot and siphon and weak expression in hepatopancreas. The mRNA expression level of ScCTL-2 in gills and hepatopancreas was significantly upregulated at 6 and 12 h after challenge with the pathogen Vibrio parahaemolyticus. The recombinant ScCTL-2 showed specific binding and agglutinate capacities to all examined Gram-negative bacterial species, namely, Escherichia coli, Vibro anguillarum, and V. parahaemolyticus in a Ca2+-independent manner. However, these binding activities were not detected in Gram-positive Micrococcus luteus. Our results indicated that ScCTL-2 could be a novel pattern recognition receptor that can specifically recognize Gram-negative microorganisms in the innate immunity of S. constricta.
机译：C型凝集素（CTL）是通过特异性识别来自病原体的碳水化合物的细菌结合和凝集的重要模式识别分子。在该研究中，从Sinonovacula Crantricta（指定的SCCTL-2）克隆了CTL的全长cDNA。 SCCTL-2的长度为981bp，47bp的5'非翻译区（UTR），3'-UTR为37bp，以及894bp的开放阅读框（ORF），它们编码了多肽298个氨基酸残基。 SCCT1-2的推导氨基酸具有类似于C-31-E-171的保守碳水化合物 - 识别结构域（CRD）。空间分布分析证明，SCCTL-2在所有测试组织中组成型表达，患有脚下的显性表达和肝胆囊中的弱表达。在与病原体vibrio parahaemolyticus攻击后6和12 h在6和12 h中显着上调SCCTL-2的mRNA表达水平。重组SCCTL-2向所有检测的革兰氏阴性细菌种类，即大肠杆菌，vibro Anguillarum和V.乙酰氨基溶解在Ca2 +彼依赖的情况下表达了特异性结合和凝集能力。然而，这些结合活性未在革兰氏阳性的微球体中检测到。我们的结果表明，SCCTL-2可以是一种新型模式识别受体，可以在S. constricta的先天免疫中具体识别革兰氏阴性微生物。

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