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首页> 外文期刊>Fish & Shellfish Immunology >pol-miR-194a of Japanese flounder (Paralichthys olivaceus) suppresses type I interferon response and facilitates Edwardsiella tarda infection
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pol-miR-194a of Japanese flounder (Paralichthys olivaceus) suppresses type I interferon response and facilitates Edwardsiella tarda infection

机译：Pol-mir-194a的日本比目鱼（Paralichthys olivaceus）抑制I型干扰素反应，并促进Edwardsiella Tarda感染

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摘要

MicroRNAs (miRNAs) are a type of small non-coding RNAs that participate in diverse cellular processes including microbial invasion and immune defense. In a previous study, we identified a large amount of Japanese flounder (Paralichthys olivaceus) miRNAs responsive to megalocytivirus infection. In the present study, we examined the function of one of these miRNAs, pol-miR-194a, in association with the infectivity of Edwardsiella tarda, an intracellular bacterial pathogen to many fish species including flounder. We found that pol-miR-194a was induced in expression to a significant extent in the spleen, liver, and gill of Japanese flounder infected by E. tarda. Transfection of flounder cells with pol-miR-194a mimic significantly enhanced the intracellular replication of E. tarda. pol-miR-194a was able to interact specifically with the 3'UTR of IRF7 in a negative manner, resulting in inhibition of IRF7 expression. Consistently, pol-miR-194a significantly blocked the promoter activity of type I interferon. Taken together, these results indicate that pol-miR-194a plays an important role in the regulation of flounder immune response as well as microbial infection, and that pol-miR-194a probably serves as a target for E. tarda to manipulate and escape host immune defense.
机译：MicroRNA（miRNA）是一种小型非编码RNA，可参与多种细胞过程，包括微生物侵袭和免疫防御。在以前的一项研究中，我们鉴定了响应于壮大的疟疾感染的大量日本比目鱼（Paralichthys Olivaceus）miRNA。在本研究中，我们研究了这些MiRNA，Pol-MiR-194a中的一种的功能，与Edwarderella Tarda的感染性，细胞内细菌病原体到许多鱼类种类，包括野生群体。我们发现，在脾脏，肝脏和被E. Tarda感染的日本比目鱼的脾脏，肝脏和鳃中诱导了Pol-miR-194a。用Pol-miR-194a模仿对比目鱼细胞的转染显着增强了E. tarda的细胞内复制。 POL-MIR-194A能够以负面方式与IRF7的3'UTR特别地相互作用，导致IRF7表达的抑制。始终如一地，POL-MIR-194A显着阻断了I型干扰素的启动子活性。这些结果表明，Pol-miR-194a在调节比较免疫应答以及微生物感染中起重要作用，并且Pol-mir-194a可能是塔拉的目标，以操纵和逃脱主持人免疫防御。

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