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首页> 外文期刊>Fish & Shellfish Immunology >Immune recognition, antimicrobial and opsonic activities mediated by a sialic acid binding lectin from Ruditapes philippinarum
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Immune recognition, antimicrobial and opsonic activities mediated by a sialic acid binding lectin from Ruditapes philippinarum

机译：免疫识别，抗菌剂和Opsonic活性由Ruditapes菲律宾的唾液酸结合凝集素介导

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In the present study, a sialic acid-binding lectin was identified and characterized from Manila clam Ruditapes philippinarum (designed as RpSABL-1). Multiple alignments strongly suggested that RpSABL-1 was a new member of the sialic acid-binding lectin family. In non-stimulated clams, RpSABL-1 transcripts were constitutively expressed in all five tested tissues, especially in hepatopancreas. After Vibrio anguillarum challenge, the expression of RpSABL-1 mRNA was significantly up-regulated at 6 h (P 0.05), 12 h (P 0.01) and 24 h (P 0.01). Recombinant RpSABL-1 protein (rRpSABL-1) displayed apparent binding activities towards lipopolysaccharides (LPS) and peptidoglycan (PGN), but not to glucan or chitin in vitro. Coinciding with the PAMPs binding assay, rRpSABL-1 exhibited obvious agglutination activities against Gram-positive bacterium Staphyloccocus aureus, Gram-negative bacteria Escherichia coli, V. anguillarum and Vibrio harveyi. Meanwhile, rRpSABL-1 showed antibacterial activities against E. coli, and biofilm formation of E. coli could also be inhibited after incubated with rRpSABL-1. Moreover, the encapsulation, phagocytosis and chemotactic ability of hemocytes could be enhanced by rRpSABL-1. All these results suggested that RpSABL-1 could function as a pattern recognition receptor with versatile functions in the innate immune responses of R. philippinarum.
机译：在本研究中，鉴定了唾液酸结合凝集素，并用Manila Clam Ruditapes Philippinarum（设计为rpsabl-1）。强烈对准表明Rpsabl-1是唾液酸结合凝集素家族的新成员。在非刺激的蛤蜊中，在所有五种测试的组织中，rpsabl-1转录物组成思考，特别是在肝癌中。在Anguillarum攻击之后，在6小时（P <0.05），12小时（P <0.01）和24小时（P <0.01）中，RPSaBl-1 mRNA的表达显着上调。重组rpsabl-1蛋白（Rrpsabl-1）显示出脂多糖（LPS）和肽聚糖（PGN）的明显结合活性，但不含葡聚糖或甲壳素体外。与Pamps结合测定重合，Rrpsabl-1对革兰氏阳性细菌金黄色葡萄球菌，革兰氏阴性细菌大肠杆菌，V.Anuillarum和Vibrio Harveyi进行了明显的凝集活性。同时，Rrpsabl-1显示针对大肠杆菌的抗菌活性，并且在与Rrpsabl-1温育后，也可以抑制大肠杆菌的生物膜形成。此外，RRPSAB1-1可以增强血细胞的包封，吞噬作用和趋化能力。所有这些结果表明，Rpsabl-1可以用作具有多功能功能的模式识别受体在R. Philippinarum的先天免疫应答中。

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