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首页> 外文期刊>Fish & Shellfish Immunology >Molecular characterization, expression and functional analysis of cystatin C in Japanese flounder (Paralichthys olivaceus)
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Molecular characterization, expression and functional analysis of cystatin C in Japanese flounder (Paralichthys olivaceus)

机译：日本比目鱼中胱抑素C的分子表征，表达和功能分析（Paralichthys Olivaceus）

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摘要

Cystatins are natural tight-binding reversible inhibitors of cysteine proteases found in a wide arrange of organisms. Studies have shown that cystatins play important roles under both physiological and pathological conditions in mammals. However, much less is known about fish cystatins. In this study, we described the identification and analysis of the gene encoding cystatin C in Japanese flounder (Paralichthys olivaceus). This gene had a high homology with the sequence of cystatin C in many fish species and had a signal peptide and three conserved functional sites. The results of qRT-PCR showed that the gene was highly expressed in the liver. Lipopolysaccharide, peptidoglycan and polyinosinic-polycytidylic acid all increased its expression after stimulation. Functional analysis showed that the recombinant P. olivaceus cystatin C purified from Escherichia coil had cysteine protease inhibitory activity and could inhibit bacterial growth by binding to bacteria. Meanwhile, rPocystatin C could up-regulate the expression of cytokines tumor necrosis factor a and interleukin 10. These results indicated that cystatin C of P. olivaceus might be considered to have the similar immunomodulatory function to mammalian cystatin.
机译：胱抑素是在广泛安排的生物体中发现的半胱氨酸蛋白酶的自然紧密可逆抑制剂。研究表明，在哺乳动物中的生理和病理条件下，胱抑素在生理和病理条件下起重要作用。然而，关于鱼胱抑素是众所周知的更少。在本研究中，我们描述了日本群体（Paralichthys Olivaceus）编码胱抑素C基因的鉴定和分析。该基因与许多鱼类中的胱抑素C序列具有高同源性，并且具有信号肽和三个保守的功能位点。 QRT-PCR的结果表明该基因在肝脏中高度表达。脂多糖，肽聚糖和多胞多环酸均在刺激后增加其表达。功能分析表明，从大肠杆菌液中纯化的重组P. Olivaceus cystatinc具有半胱氨酸蛋白酶抑制活性，并且可以通过与细菌结合来抑制细菌生长。同时，rpocystatin c可以上调细胞因子肿瘤坏死因子A和白细胞介素10.这些结果表明，P. Olivaceus的胱抑素C可能被认为具有与哺乳动物胱抑素的类似免疫调节功能。

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  • 来源
    《Fish & Shellfish Immunology》 |2019年第2019期|共7页
  • 作者

    Yu Haiyang; Xu Xin; Zhang Quanqi; Wang Xubo;

  • 作者单位

    Ocean Univ China Minist Educ Key Lab Marine Genet &

    Breeding Qingdao 266003 Shandong Peoples R China;

    Ocean Univ China Minist Educ Key Lab Marine Genet &

    Breeding Qingdao 266003 Shandong Peoples R China;

    Ocean Univ China Minist Educ Key Lab Marine Genet &

    Breeding Qingdao 266003 Shandong Peoples R China;

    Ocean Univ China Minist Educ Key Lab Marine Genet &

    Breeding Qingdao 266003 Shandong Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 水产、渔业;
  • 关键词

    Paralichthys olivaceus; Cystatin C; Immune response; Inhibit bacteria;

    机译：paralichthys olivaceus;胱抑素c;免疫应答;抑制细菌;

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