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首页> 外文期刊>Fish & Shellfish Immunology >The immune response of the scallop Argopecten purpuratus is associated with changes in the host microbiota structure and diversity
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The immune response of the scallop Argopecten purpuratus is associated with changes in the host microbiota structure and diversity

机译：扇贝argopecten purpuratus的免疫应答与宿主微生物群结构和多样性的变化有关

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摘要

All organisms live in close association with a variety of microorganisms called microbiota. Furthermore, several studies support a fundamental role of the microbiota on the host health and homeostasis. In this context, the aim of this work was to determine the structure and diversity of the microbiota associated with the scallop Argopecten purpuratus, and to assess changes in community composition and diversity during the host immune response. To do this, adult scallops were immune challenged and sampled after 24 and 48 h. Activation of the immune response was established by transcript overexpression of several scallop immune response genes in hemocytes and gills, and confirmed by protein detection of the antimicrobial peptide big defensin in gills of Vibrio-injected scallops at 24 h post-challenge. Then, the major bacterial community profile present in individual scallops was assessed by denaturing gradient gel electrophoresis (DGGE) of 16S rDNA genes and dendrogram analyses, which indicated a clear Glade differentiation of the bacterial communities noticeable at 48 h post-challenge. Finally, the microbiota structure and diversity from pools of scallops were characterized using 16S deep amplicon sequencing. The results revealed an overall modulation of the microbiota abundance and diversity according to scallop immune status, allowing for prediction of some changes in the functional potential of the microbial community. Overall, the present study showed that changes in the structure and diversity of bacterial communities associated with the scallop A. purpuratus are detected after the activation of the host immune response. Now, the relevance of microbial balance disruption in the immune capacity of the scallop remains to be elucidated.
机译：所有生物体与各种称为Microbiota的微生物密切相关。此外，一些研究支持微生物群对宿主健康和稳态的基本作用。在这种情况下，这项工作的目的是确定与扇贝argopecten purpuratus相关的微生物群的结构和多样性，并在宿主免疫应答期间评估群落成分和多样性的变化。为此，成人扇贝在24至48小时后受到挑战和取样的免疫挑战和取样。通过血细胞和鳃中的几种扇贝免疫应答基因的转录物过表达来确定免疫反应的激活，并在攻击后24小时的24小时内通过蛋白质检测抗微生物肽大防死素的蛋白质检测。然后，通过使16S rDNA基因的梯度凝胶电泳（DGGE）和树木分析的梯度凝胶电泳（DGGE）进行评估，评估各种扇贝中存在的主要细菌群落分析，这表明在攻击后48小时内明显的细菌群落的透明林间界分。最后，使用16S深度扩增子测序来表征扇贝池中的微生物群结构和多样性。结果表明，根据扇贝免疫地位，允许预测微生物群落功能潜力的一些变化的微生物群丰度和多样性的总体调节。总体而言，本研究表明，在激活宿主免疫应答后检测到与扇贝A.Purpuratus相关的细菌社区结构和多样性的变化。现在，微生物平衡中断在扇贝的免疫能力中的相关性仍有待阐明。

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