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首页> 外文期刊>Fish & Shellfish Immunology >The effects of benzo[a]pyrene on the composition of gut microbiota and the gut health of the juvenile sea cucumber Apostichopus japonicus Selenka
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The effects of benzo[a]pyrene on the composition of gut microbiota and the gut health of the juvenile sea cucumber Apostichopus japonicus Selenka

机译：苯并[A]芘对肠道微生物组成的影响及少年海洋黄瓜Apostichopus japonicus selenka的肠道健康

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摘要

The gut microbiota is essential for health and physiological functions in the host organism. However, the toxicological evaluation of environmental pollutants on the gut microbiota is still insufficient. In the present study, the juvenile sea cucumber Apostichopus japonicus was exposed for 14 days to Benzo[a]pyrene (BaP), which is a model polycyclic aromatic hydrocarbon (PAH), at four different concentrations (0, 0.5, 5, and 25 mu g/L). We analyzed the intestinal microbial community of A. japonicas using 16S rRNA gene amplicon sequencing. Our results demonstrate that BaP exposure caused alterations to the microbiome community composition in sea cucumbers. At the phylum level, Planctomycetes were significantly more abundant in BaP exposure groups at 14 d compared with the control group, and the abundance of Proteobacteria and Bacteroidetes increased while the abundance of Firmicutes decreased following BaP exposure. At the genus level, multiple beneficial and autochthonous genera declined in the BaP treatment groups compared to the control, including Lactococcus, Bacillus, Lactobacillus, Enterococcus, Leuconostoc and Weissella; however, a bloom of alkane-degrading bacteria was found in BaP-exposed guts and included Lutibacter, Pseudoalteromonas, Polaribacter, Rhodopirellula and Blastopirellula. Furthermore, histological morphology, enzymatic activity and gene expression analysis revealed that BaP exposure also negatively impacted gut structure and function and presented as inflammation or atrophy, oxidative stress and immune suppression in sea cucumber intestines. Collectively, these findings provide insights into the toxic effects of BaP exposure on A. japonicas associated with intestinal microbiota and health.
机译：肠道微生物酵母对宿主生物体的健康和生理功能至关重要。然而，肠道微生物群环境污染物的毒理学评估仍然不足。在本研究中，少年海洋黄瓜己酮血吸虫将粳稻暴露于苯并[a]芘（Bap），其是多环芳烃（PAH），四种不同浓度（0,0.5,5和25 mu g / l）。我们使用16S rRNA基因扩增子测序分析了A. japonicas的肠道微生物群落。我们的结果表明，BAP暴露导致海参中微生物组成的变化。在门水平下，与对照组相比，14d的BAP暴露基团中的平分菌在14d中显着越来越丰富，并且植物杆菌和细菌的丰度增加，而BAP暴露后的额度降低。在水平，与对照组相比，乳酸乳杆菌，芽孢杆菌，乳杆菌，肠球菌，leuconostoc和Weissella相比，在BAP治疗组中，多种有益和自身加热的属于含有多种有益和自身加热的属。然而，在甲烷暴露的肠道中发现了含烷烃降解细菌的盛开，包括Lutibacter，假核酸，偏振杆菌，乳菌细胞和囊皮细胞。此外，组织学形态，酶活性和基因表达分析表明，BAP暴露也受到肠道结构的负面影响，并且作为炎症或萎缩，氧化应激和海洋黄瓜肠中的免疫抑制。集体，这些发现提供了对与肠道微生物群系和健康相关的A.粳稻的毒性作用的见解。

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  • 来源
    《Fish & Shellfish Immunology》 |2019年第2019期|共11页
  • 作者

    Zhao Ye; Liu Hui; Wang Qing; Li Bingjun; Zhang Hongxia; Pi Yongrui;

  • 作者单位

    Yantai Univ Ocean Sch Yantai 264005 Peoples R China;

    Chinese Acad Sci Yantai Inst Coastal Zone Res Res &

    Dev Ctr Efficient Utilizat Coastal Bioresou Yantai Peoples R China;

    Chinese Acad Sci Yantai Inst Coastal Zone Res Res &

    Dev Ctr Efficient Utilizat Coastal Bioresou Yantai Peoples R China;

    Yantai Univ Ocean Sch Yantai 264005 Peoples R China;

    Chinese Acad Sci Yantai Inst Coastal Zone Res Res &

    Dev Ctr Efficient Utilizat Coastal Bioresou Yantai Peoples R China;

    Yantai Univ Ocean Sch Yantai 264005 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 水产、渔业;
  • 关键词

    Benzoapyrene; Sea cucumber; Gut microbiota; 16S rRNA; Gene sequencing;

    机译：Benzo [a]芘;海参;肠道微生物;16s rRNA;基因测序;

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