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首页> 外文期刊>Fish & Shellfish Immunology >Oxidative stress, apoptosis activation and symbiosis disruption in giant clam Tridacna crocea under high temperature
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Oxidative stress, apoptosis activation and symbiosis disruption in giant clam Tridacna crocea under high temperature

机译：高温下巨型蛤激曲线鳄鱼氧化应激，凋亡激活和共生破坏

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摘要

Giant clams are one of the most important animals in coral reef ecosystem, and its growth and reproduction are being threatened by heat stress due to global warming. In the present study, the symbiont density, the crucial enzyme activities and the transcriptome were investigated in the outer mantle of giant clam Tridacna crocea after the acute exposure of high temperature. The density of symbiotic zooxanthellae decreased significantly during 12-24 h, with the minimum level (7.75 x 10(5) cell cm(-2), p & 0.05) at 12 h after heat stress. The activities of superoxide dismutase in the heat stress group was significantly lower than that in the control group at 24 h after heat stress, while no significant change in the activities of catalase was observed during the entire stress process. The activation level of caspase3 began to increase significantly at 12 h (1.22-fold, p & 0.05), and reached the highest level at 24h (1.38-fold, p & 0.05) after heat stress. Six paired-end libraries were sequenced in two groups, including the heat stress and control group at 12 h after heat stress. Through the assembling of 187,116,632 paired-end reads with lengths of 2 x 150 bp, a total of 26,676 genes were obtained which derived from giant dam. Bioinformatics analysis revealed 47 significantly upregulated and 88 significantly downregulated genes at 12 h after the treatment. There were 12 overrepresented GO terms for significantly upregulated genes, mostly related to unfolded protein binding and ATP binding, whereas no GO term was overrepresented for significantly downregulated genes. These results collectively suggest high temperature could induce excessive oxidative stress through the repressed antioxidant ability, the apoptosis activated by the unfolded protein response, and further the collapse of the symbiosis between host and symbiont, which has been threatening the growth and reproduction of the giant clam T. crocea.
机译：巨型蛤是珊瑚礁生态系统中最重要的动物之一，其增长和繁殖是由于全球变暖而受到热压力的威胁。在本研究中，在急性暴露的高温暴露后，在巨型蛤TridacnaCrocea的外部地幔中研究了Symbiont密度，关键酶活性和转录组。共生Zooxanthellae的密度在12-24小时内显着降低，最小水平（7.75×10（5）个细胞cm（-2），p＆amp; 0.05）在热应激后12小时。在热应激后24小时，热应激组中超氧化物歧化酶的活性显着低于对照组，同时在整个应力过程中观察到过氧化氢酶活性的显着变化。 CasPase3的活化水平开始于12小时（1.22倍，P＆amp; 0.05）显着增加，并在热应激后在24小时（1.38倍，P＆amp; LT; 0.05）达到最高水平。在两组中测序六个成对终端文库，在热应激后12小时内包括热应激和对照组。通过组装187,116,632对末端的读数，长度为2×150bp，得到总共26,676个基因，衍生自巨大坝。生物信息学分析显示47显着上调，治疗后12小时显着下调和88个显着下调的基因。对于显着上调的基因，有12个过度持续的GO术语，主要与展开的蛋白质结合和ATP结合有关，而没有过度术语以显着下调的基因过高。这些结果共同提出了通过抑制抗氧化能力的高温，通过展开蛋白质反应激活的细胞凋亡，以及宿主和酶学性之间的共生崩溃，这一直威胁到巨型蛤蜊之间的共生崩溃T. Crocea。

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  • 来源
    《Fish & Shellfish Immunology》 |2019年第2019期|共7页
  • 作者

    Zhou Zhi; Liu Zhaoqun; Wang Lingui; Luo Jian; Li Hailang;

  • 作者单位

    Hainan Univ State Key Lab Marine Resource Utilizat South Chin Haikou 570228 Hainan Peoples R China;

    Dalian Ocean Univ Liaoning Key Lab Marine Anim Immunol &

    Dis Contro Dalian 116023 Peoples R China;

    Hainan Univ State Key Lab Marine Resource Utilizat South Chin Haikou 570228 Hainan Peoples R China;

    Hainan Univ State Key Lab Marine Resource Utilizat South Chin Haikou 570228 Hainan Peoples R China;

    Haikou 4 Middle Sch Haikou 570203 Hainan Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 水产、渔业;
  • 关键词

    Heat stress; Oxidative stress; Apoptosis; Tridacna; Symbiosis;

    机译：热应激;氧化应激;细胞凋亡;Tridacna;共生;

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