...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Fish & Shellfish Immunology >A histone H2A derived antimicrobial peptide, Fi-Histin from the Indian White shrimp, Fenneropenaeus indicus: Molecular and functional characterization
【24h】

A histone H2A derived antimicrobial peptide, Fi-Histin from the Indian White shrimp, Fenneropenaeus indicus: Molecular and functional characterization

机译：来自印度白虾，Fenneropenaeus的组蛋白H2a衍生的抗微生物肽，Fe-histin inclicus：分子和功能表征

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Antimicrobial peptides (AMPS) derived from histone proteins form an important category of peptide antibiotics. Present study deals with the molecular and functional characterization of a 27-amino acid histone H2A derived AMP from the Indian White shrimp, Fenneropenaeus indicus designated as Fi-Histin. This peptide displayed distinctive features of AMPS such as amphiphilic alpha helical structure and a net charge of +6. The synthetic peptide exhibited significant antimicrobial activity against Gram-negative and Gram-positive bacteria especially against V. vulnificus, P. aeruginosa, V. parahaemolyticus, V. cholera and S. aureus. Disruption of cell membrane and cell content leakage were observed in peptide treated V. vulnificus using scanning electron microscopy. The synthetic peptide Fi-His(1-21) exhibited DNA binding activity and found to be non-haemolytic at the tested concentrations. Peptide was also found to possess anticancer activity against NCI-H460 and HEp-2 cell lines with an IC50 of 22.670 +/- 13.939 mu M and 31.274 +/- 24.531 mu M respectively. This is the first report of a histone H2A derived peptide from F. indicus with a specific antimicrobial activity and anticancer activity, which could be a new candidate for future applications in aquaculture and medicine.
机译：衍生自组蛋白蛋白的抗微生物肽（AMPs）形成了一类重要的肽抗生素。本研究涉及来自印度白虾的27-氨基酸组蛋白H2A衍生AMP的分子和功能表征，Fenneropenaeus指定为FI- Histin。该肽显示了诸如两亲α螺旋结构等AMPS的独特特征和+6的净电荷。合成肽对革兰氏阴性和革兰氏阳性细菌表现出显着的抗微生物活性，尤其是针对V.Vulnificus，P.Fulginosa，V.Parahaemolyticus，V.霍乱和金黄色葡萄球菌。使用扫描电子显微镜观察在肽处理的V.Vulnfificus中观察细胞膜和细胞含量泄漏的破坏。合成肽Fi-His（1-21）表现出DNA结合活性，发现在测试浓度下是非溶血性的。还发现肽具有针对NCI-H460和HEP-2细胞系的抗癌活性，IC50分别为22.670 +/- 13.939 mu m和31.274 +/- 24.531 mu m。这是来自F. Indicus的组蛋白H2A衍生肽的第一个报告，具有特异性抗微生物活性和抗癌活动，这可能是水产养殖和医学中未来应用的新候选者。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号