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首页> 外文期刊>Fish & Shellfish Immunology >Transcriptomic analysis of clam extrapallial fluids reveals immunity and cytoskeleton alterations in the first week of Brown Ring Disease development
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Transcriptomic analysis of clam extrapallial fluids reveals immunity and cytoskeleton alterations in the first week of Brown Ring Disease development

机译：CLAM挤压液的转录组分析揭示了棕色环疾病发育第一周的免疫力和细胞骨架改变

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The Brown Ring Disease is an infection caused by the bacterium Vibrio tapetis on the Manila clam Ruditapes philippinarum. The process of infection, in the extrapallial fluids (EPFs) of clams, involves alteration of immune functions, in particular on hemocytes which are the cells responsible of phagocytosis. Disorganization of the actincytoskeleton in infected clams is a part of what leads to this alteration. This study is the first transcriptomic approach based on collection of extrapallial fluids on living animals experimentally infected by V. tapetis. We performed differential gene expression analysis of EPFs in two experimental treatments (healthy-against infectedclams by V. tapetis), and showed the deregulation of 135 genes. In infected clams, a downregulation of transcripts implied in immune functions (lysosomal activity and complement- and lectin-dependent PRR pathways) was observed during infection. We also showed a deregulation of transcripts encoding proteins involved in the actin cytoskeleton organization such as an overexpression of beta 12-Thymosin (which is an actin sequestration protein) or a downregulation of proteins that closely interact with capping proteins such as Coactosin, that counteract action of capping proteins, or Profilin. We validated these transcriptomic results by cellular physiological analyses that showed a decrease of the lysosome amounts and the disorganization of actin cytoskeleton in infected hemocytes.
机译：棕色戒指疾病是由Manila Clam Ruditapes Philippinarum的细菌Vibrio Tapetis引起的感染。在蛤蜊的挤压液（EPFS）中感染过程涉及免疫功能的改变，特别是对血细胞的血细胞，这是负责吞噬作用的细胞。感染蛤蜊的ATTINCYTOSKELEON的紊乱是导致这种改变的一部分。本研究是基于由V.Papetis实验感染的活体动物的挤压液收集的第一种转录组方法。我们在两种实验治疗中对EPFS进行了差异基因表达分析（通过V.Gingetis的健康反对感染Clams），并显示了135个基因的放松管制。在感染的蛤蜊中，在感染期间观察到在免疫功能（溶酶体活性和补素和依赖素依赖性PRR途径）中暗示的转录物的下调。我们还展示了编码参与肌动蛋白细胞骨架组织的蛋白质的转录物的病程，例如β12-胸苷（其是肌动蛋白螯合蛋白）的过度表达或蛋白质的下调，其与封端蛋白如Caactosin密切相关，即抵消作用封端蛋白或profilin。我们通过细胞生理分析验证了这些转录组结果，所述细胞生理分析显示出溶酶体量的降低以及感染血细胞中肌动蛋白细胞骨架的紊乱。

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