The Penaeus stylirostris densovirus capsid interacts with Litopenaeus vannamei troponin I

Chen Xiuli; Zeng Digang; Zhu Weilin; Peng Min; Yang Chunling; Li Qiangyong; Liu Qingyun; Yang Qiong; Wang Hui; Li Min; Lin Yong; Zhao Yongzhen; Chen Xiaohan

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The Penaeus stylirostris densovirus capsid interacts with Litopenaeus vannamei troponin I

机译：penaeus tylyostris densovirus capsid与litopenaeus vannamei肌钙蛋白I相互作用

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The Penaeus stylirostris densovirus (PstDNV) (also known as infectious hypodermal and hematopoietic necrosis virus, IHHNV), a very small DNA virus, is a major shrimp pathogen. The PstDNV genome encodes only two nonstructural proteins and one capsid protein. This virus is thus an ideal, simple model for the investigation of virus-host interactions. To explore the role of the PstDNV capsid in viral infections, a yeast two-hybrid (Y2H) cDNA library was constructed based on Pacific white shrimp, Litopenaeus vannamei mRNA. The Y2H library was then screened, using the PstDNV capsid protein as bait. We identified a host protein that interacted strongly with the PstDNV capsid as L. vannamei troponin I (LvTnI). An in vitro co-immunoprecipitation experiment further supported this interaction. In addition, an in vivo neutralization experiment showed that the vaccination with anti-LvTnI significantly reduced PstDNV copies in PstDNV-challenged shrimp, indicating that the interaction between the PstDNV capsid and cellular LvTnI is essential for PstDNV infection. This result has important implications for our understanding of the mechanisms by which PstDNV infects shrimp.

机译：PenaeusTyrostris Densovirus（PSTDNV）（也称为传染性皮下病和造血坏死病毒，IHHNV），非常小的DNA病毒是一个主要的虾病原体。 PSTDNV基因组仅编码两个非结构蛋白和一个衣壳蛋白。因此，该病毒是一种理想的简单模型，用于调查病毒 - 宿主相互作用。为了探讨Pstdnv衣壳在病毒感染中的作用，基于太平洋白虾，Litopenaeus Vannamei mRNA构建了酵母双杂交（Y2H）cDNA文库。然后使用PstDNV Capsid蛋白作为诱饵筛选Y2H文库。我们鉴定了一种宿主蛋白，其与PSTDNV胶囊强烈相互作用，如L.Vannamei肌钙蛋白I（LVTNI）。体外共热沉淀实验进一步支持该相互作用。此外，体内中和实验表明，抗LVTNI的疫苗接种显着降低了PstDNV攻击虾中的PSTDNV拷贝，表明PSTDNV衣壳和细胞LVTNI之间的相互作用对于PSTDNV感染是必不可少的。这一结果对我们对Pstdnv感染虾的机制的理解具有重要意义。

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来源
《Fish & Shellfish Immunology》 |2019年第2019期|共6页
作者
Chen Xiuli; Zeng Digang; Zhu Weilin; Peng Min; Yang Chunling; Li Qiangyong; Liu Qingyun; Yang Qiong; Wang Hui; Li Min; Lin Yong; Zhao Yongzhen; Chen Xiaohan;
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作者单位

Guangxi Acad Fisheries Sci Guangxi Key Lab Aquat Genet Breeding &

Hlth Aquac Nanning Peoples R China;

Guangxi Acad Fisheries Sci Guangxi Key Lab Aquat Genet Breeding &

Hlth Aquac Nanning Peoples R China;

Guangxi Acad Fisheries Sci Guangxi Key Lab Aquat Genet Breeding &

Hlth Aquac Nanning Peoples R China;

Guangxi Acad Fisheries Sci Guangxi Key Lab Aquat Genet Breeding &

Hlth Aquac Nanning Peoples R China;

Guangxi Acad Fisheries Sci Guangxi Key Lab Aquat Genet Breeding &

Hlth Aquac Nanning Peoples R China;

Guangxi Acad Fisheries Sci Guangxi Key Lab Aquat Genet Breeding &

Hlth Aquac Nanning Peoples R China;

Guangxi Acad Fisheries Sci Guangxi Key Lab Aquat Genet Breeding &

Hlth Aquac Nanning Peoples R China;

Guangxi Acad Fisheries Sci Guangxi Key Lab Aquat Genet Breeding &

Hlth Aquac Nanning Peoples R China;

Guangxi Acad Fisheries Sci Guangxi Key Lab Aquat Genet Breeding &

Hlth Aquac Nanning Peoples R China;

Guangxi Acad Fisheries Sci Guangxi Key Lab Aquat Genet Breeding &

Hlth Aquac Nanning Peoples R China;

Guangxi Acad Fisheries Sci Guangxi Key Lab Aquat Genet Breeding &

Hlth Aquac Nanning Peoples R China;

Guangxi Acad Fisheries Sci Guangxi Key Lab Aquat Genet Breeding &

Hlth Aquac Nanning Peoples R China;

Guangxi Acad Fisheries Sci Guangxi Key Lab Aquat Genet Breeding &

Hlth Aquac Nanning Peoples R China;

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正文语种 eng
中图分类水产、渔业;
关键词
Penaeus stylirostris densovirus; Capsid; Interaction; Litopenaeus vannamei; Troponin I;

机译：penaeus stylirostris densovirus;capsid;互动;litopenaeus vannamei;肌钙蛋白我;

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专利

1. Single or combined effects of dietary arabinoxylan-oligosaccharide and inulin on growth performance, gut microbiota, and immune response in Pacific white shrimp Litopenaeus vannamei [J] . Yun LI, Wei YUAN, Yi ZHANG, 中国海洋湖沼学报（英文版） . 2021,第002期
2. Single or combined effects of dietary arabinoxylan-oligosaccharide and inulin on growth performance,gut microbiota,and immune response in Pacific white shrimp Litopenaeus vannamei [J] . Yun LI, Wei YUAN, Yi ZHANG, 海洋湖沼学报（英文） . 2021,第002期
3. The Penaeus stylirostris densovirus capsid interacts with Litopenaeus vannamei troponin I [J] . Chen Xiuli, Zeng Digang, Zhu Weilin, Fish & Shellfish Immunology . 2019,第期

机译：penaeus tylyostris densovirus capsid与litopenaeus vannamei肌钙蛋白I相互作用
4. Double-stranded RNA confers both preventive and therapeutic effects against Penaeus stylirostris densovirus (PstDNV) in Litopenaeus vannamei. [J] . Ho T, Yasri P, Panyim S, Virus Research: An International Journal of Molecular and Cellular Virology . 2011,第1期

机译：双链RNA赋予南美白对虾对虾对虾对虾病毒（PstDNV）的预防和治疗作用。
5. Sperm-egg incompatibility in inter-specific inseminations of Penaeus (Litopenaeus) occidentalis, P. (Litopenaeus) stylirostris and P. (Litopenaeus) vannamei [J] . Ulate Karol, Alfaro-Montoya Jorge Aquaculture . 2010,第1a4期

机译：西方对虾（Litopenaeus），西伯利亚对虾（Litopenaeus）stylirostris和南美白对虾（Litopenaeus）vannamei的种间授精中的精卵不相容性
6. EVOLUTION OF THE GENETIC VARIABILITY FOR BODY WEIGHT AT HARVEST SIZE IN A SELECTED POPULATION OF PACIFIC WHITE SHRIMP Penaeus (Litopenaeus) mnnamei [C] . Hugo H. Montaldo, Nelson Cala, Felipe de Jesus Ruiz Lopez, Annual Meeting of the European Aquaculture Society . 2013

机译：植物植物（Litopenaeus）植物群中收获大小遗传变异的进化
7. Taura syndrome virus (TSV) of penaeid shrimp: Infection of Penaeus monodon, resistance of Litopenaeus vannamei and ultrastructure of the replication site in infected cells [D] . Srisuvan, Thinnarat 2006

机译：对虾对虾Taura综合征病毒（TSV）：斑节对虾的感染，凡纳滨对虾的抗性和感染细胞中复制位点的超微结构
8. Persistence of Penaeus stylirostris densovirus delays mortality caused by white spot syndrome virus infection in black tiger shrimp (Penaeus monodon) [O] . Sudkhate Molthathong, Sarocha Jitrakorn, Yutthana Joyjinda, 2013

机译：持久性对虾对虾病毒持续存在会延缓黑虎虾白斑综合症病毒感染导致的死亡率（斑节对虾）
9. In situ hybridization demonstrates that Litopenaeus vannamei, L. stylirostris and Penaeus monodon are susceptible to experimental infection with infectious myonecrosis virus (IMNV) [O] . KFJ Tang, CR Pantoja, BT Poulos, 2005

机译：原位杂交表明，Litopenaeus Vannamei，L.Byteirostris和Penaeus·莫登易受传染性肌坏死病毒（IMNV）的实验感染的影响

The Penaeus stylirostris densovirus capsid interacts with Litopenaeus vannamei troponin I

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