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首页> 外文期刊>Fish & Shellfish Immunology >Superoxide dismutase B (sodB), an important virulence factor of Vibrio alginolyticus, contributes to antioxidative stress and its potential application for live attenuated vaccine
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Superoxide dismutase B (sodB), an important virulence factor of Vibrio alginolyticus, contributes to antioxidative stress and its potential application for live attenuated vaccine

机译：超氧化物歧化酶B（SODB）是vibrio alginyticus的重要毒力因子，有助于抗氧化应激及其潜在的活病毒疫苗施用

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摘要

Vibrio alginolyticus is an opportunistic and halophilic Gram-negative pathogen in limiting the development of aquatic industry and affecting human health. SODs are oxidative enzymes that play a critical role in oxidative defense. In this study, an in-frame deleted mutant strain (Delta sodB) was constructed by allelic exchange mutagenesis to investigate physiological role of sodB in pathogenicity of V. alginolyticus. The results exhibited that Delta sodB showed no differences in growth compared with wild-type strain HY9901 (WT), but led to increasing in biofilm formation, ECPase activity and sensitivity to hydrogen peroxide, decreasing in swarming motility, adherence to CIK cells, SOD activity and virulence. In addition, Delta sodB induced a high antibody titer and provided a valid protection with a relative percent survival value of 86.5% without inducing clinical symptoms after challenging with WT. These results suggest that sodB is important for normal physiological function, oxidation resistance and virulence in V. alginolyticus, and Delta sodB may be considered as an effective live attenuated vaccine against V. alginolyticus.
机译：Vibrio Alginolyticus是一种机会主义和嗜嗜嗜盐革兰氏阴性病原体，限制了水生行业的发展并影响人类健康。 SODS是在氧化防御中起关键作用的氧化酶。在该研究中，通过等位基因交换诱变构建了框架缺失的突变菌株（Delta SOCB），以研究SODB在V.Alginolyticus的病原质中的生理作用。结果表明，与野生型菌株HY9901（WT）相比，Delta SODB没有差异的生长，但导致生物膜形成，ECP酶活性和对过氧化氢的敏感性增加，蜂拥而至，对CIK细胞的粘附性，SOD活性依赖和毒力。此外，Delta SODB诱导高抗体滴度，并提供了有效的保护，相对百分比的生存值为86.5％，而不会在用wt挑战后诱导临床症状。这些结果表明SODB对于正常的生理功能，V.Alginolyticus中的抗氧化和毒力和Delta SODB是重要的，可以被认为是针对V.Alginolyticus的有效活化的疫苗。

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