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首页> 外文期刊>Fish & Shellfish Immunology >2-Transmembrane C-type lectin from oriental river prawn Macrobrachium nipponense participates in antibacterial immune response
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2-Transmembrane C-type lectin from oriental river prawn Macrobrachium nipponense participates in antibacterial immune response

机译：东方河虾大草菌Nippoonense的2型 - 跨膜C型凝集素参与抗菌免疫反应

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摘要

As a type of pattern-recognition proteins (PRRs), C-type lectins (CTLs) perform important functions in non-self recognition and clearance of pathogens in innate immunity. In this study, a unique 2-transmembrane CTL (designated as Mn-2TM-cLec) with a single carbohydrate recognition domain (CRD) was isolated from Macrobrachium nipponense. The full-length cDNA of Mn-2TM-cLec consisted of 3265 bp with an 837 bp open reading frame encoding a protein with 278 amino acids. Mn-2TM-cLec was ubiquitously distributed in various tissues of normal prawn, particularly in the hemocytes, hepatopancreas, and gills. The expression of Mn-2TM-cLec was significantly up-regulated in the gills and hepatopancreas after the prawns were challenged with Staphylococcus aureus and Vibrio parahaemolyticus. RNA interference knock-down of Mn-2TM-cLec gene decreased the transcription levels of three antimicrobial peptides (anti-lipopolysaccharide factor (ALF) 1, ALF2, and Crustin (Crus) 1) after V. parahaemolyticus infection. The recombinant CRD of Mn-2TM-cLec could bind lipopolysaccharide, peptidoglycans, and diverse bacterial strains and agglutinate S. aureus and V. parahaemolyticus in a Ca2+-dependent manner. In addition, the rCRD enhanced the clearance of V. parahaemolyticus injected in prawns. In summary, Mn-2TM-cLec might act as a PRR to participate in the prawn immune defense against pathogens through its antimicrobial activity.
机译：作为一种模式识别蛋白（PRRS），C型凝集素（CTL）在先天免疫中的非自我识别和清除性中进行重要作用。在本研究中，从大草菌Nippoonense中分离出具有单个碳水化合物识别结构域（CRD）的独特的2跨膜CTL（指定为MN-2TM-CLEC）。 MN-2TM-CLEC的全长cDNA由3265bp组成，具有837bp开放阅读框，编码具有278个氨基酸的蛋白质。 Mn-2TM-CLEC在正常虾的各种组织中普遍存在，特别是在血细胞，肝病和鳃中。在虾用金黄色葡萄球菌和血管乙酰氨基醇的尖塔攻击虾仁挑战后，Mn-2Tm-Clec的表达在鳃和肝胆囊中显着上调。 Mn-2TM-CLEC基因的RNA干扰倒闭降低了V.副溶解剂感染后的三种抗微生物肽（抗脂多糖因子（ALF）1，ALF2和甲壳素（CRUS）1）的转录水平。 MN-2TM-CLEC的重组CRD可以以CA2 +依赖性粘合脂多糖，肽聚糖和各种细菌菌株和凝集的S.UURESTICUS。此外，RCRD增强了在虾中注射的V.副磷酸盐的清除。总之，MN-2TM-CLEC可以作为通过其抗微生物活性参与对抗病原体的PRR。

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