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首页> 外文期刊>Fish & Shellfish Immunology >Pattern analysis of conditional essentiality (PACE)-based heuristic identification of an in vivo colonization determinant as a novel target for the construction of a live attenuated vaccine against Edwardsiella piscicida
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Pattern analysis of conditional essentiality (PACE)-based heuristic identification of an in vivo colonization determinant as a novel target for the construction of a live attenuated vaccine against Edwardsiella piscicida

机译：条件基本度（速度）的模式分析 - 基于体内殖民定期鉴定为埃迪德拉菌施工生命减毒疫苗的新靶点

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摘要

Edwardsiella piscicida is the aetiological agent of fish edwardsiellosis, causing huge economic losses in aquaculture industries. The use of a live attenuated vaccine (LAV) will be an effective strategy to control the disease in farmed fish. Thus, methods facilitating exploration of targets used for construction of an LAV will be of great significance. Previously, we devised an algorithm termed pattern analysis of conditional essentiality (PACE) to perform genome-wide analysis of the temporal dynamic behaviour of E. piscicida mutants colonizing turbot. Here, we correlated the conditional essentiality patterns of the PACE-derived colonization determinants with that of the aroC gene encoding chorismate synthase, the established target for LAV construction in E. piscicida, and identified ETAE_0023 as a novel valuable LAV target. ETAE_0023 encodes an uncharacterized DcrB family protein. Deletion of ETAE_0023 dramatically impaired E. piscicida invasion capability in ZF4 cells as well as colonization in fish and resulted in in vivo clearance at similar to 30 days post-infection. Delta ETAE_0023 showed an similar to 2500-fold higher 50% lethal dose (LD50) than that of the wild type strain. Vaccination with Delta ETAE_0023 by intraperitoneal (i.p.) injection upregulated expression of immune factors, i.e., IL-1 beta, IgM, MHC-I and MHC-II, and produced significantly high levels of E. piscicida-specific IgM as well as serum bactericidal capacities in turbot. Moreover, a single i.p. inoculation with Delta ETAE_0023 generated significant protection comparable to the established WED LAV strain in turbot against challenge with the wild type strain after 5 weeks of vaccination. Taken together, we demonstrated a PACE-based method for heuristic identification of targets for LAV construction and presented Delta ETAE_0023 as a new LAV candidate against edwardsiellosis.
机译：Edwardsiella piscicida是鱼Edwardiellis的终止剂，在水产养殖行业导致巨大的经济损失。使用活病毒疫苗（LAV）将是控制养殖鱼类疾病的有效策略。因此，促进探索用于建造熔炉的目标的方法将具有重要意义。以前，我们设计了一种算法称为条件基本度（速度）的算法进行了模式分析，以进行大规模殖民大菱粒子的临时动态行为的基因组分析。在这里，我们将阶段衍生的定子决定簇的条件基本性模式与编码酸酸合酶的芳香基因基因的条件基本程度相关，该靶标在大型碱中的LAV结构靶标，并鉴定为新型宝贵的LAV目标。 Etae_0023编码一个无表达DCRB家族蛋白质。缺失的Etae_0023在ZF4细胞中大大受损，ZF4细胞中的侵袭能力以及鱼类的定植，并导致感染后30天类似的空间间隙。 Delta etae_0023显示出与野生型菌株的50％致命剂量（LD50）类似于2500倍。通过腹膜内（IP）注射ΔEtae_0023接种免疫因子的上调表达，即IL-1β，IgM，MHC-I和MHC-II，并产生显着高水平的大肠碱特异性IgM以及血清杀菌剂轰轰烈斯特的能力。而且，单个I.P.与Delta etae_0023接种产生的显着保护与湍流中的建立的与阵列与野生型菌株疫苗接种后的攻击攻击相当。我们一起展示了一种基于步伐的稳定性识别厕所识别，并将Delta eTae_0023作为反对Edwardsiellosis的新的LAV候选者呈现。

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  • 来源
    《Fish & Shellfish Immunology》 |2019年第2019期|共8页
  • 作者

    Ma Ruiqing; Yang Guanhua; Xu Rongjing; Liu Xiaohong; Zhang Yuanxing; Ma Yue; Wang Qiyao;

  • 作者单位

    East China Univ Sci &

    Technol State Key Lab Bioreactor Engn Shanghai 200237 Peoples R China;

    East China Univ Sci &

    Technol State Key Lab Bioreactor Engn Shanghai 200237 Peoples R China;

    Yantai Tianyuan Aquat Co Ltd Yantai Shandong Peoples R China;

    East China Univ Sci &

    Technol State Key Lab Bioreactor Engn Shanghai 200237 Peoples R China;

    East China Univ Sci &

    Technol State Key Lab Bioreactor Engn Shanghai 200237 Peoples R China;

    East China Univ Sci &

    Technol State Key Lab Bioreactor Engn Shanghai 200237 Peoples R China;

    East China Univ Sci &

    Technol State Key Lab Bioreactor Engn Shanghai 200237 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 水产、渔业;
  • 关键词

    Edwardsiella piscicida; In vivo colonization; Live attenuated vaccine; PACE;

    机译：Edwardsiella piscicida;体内定植;现场减毒疫苗;节奏;

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