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首页> 外文期刊>Fish & Shellfish Immunology >Functional identification and expressional responses of large yellow croaker (Larimichthys crocea) interleukin-8 and its receptor
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Functional identification and expressional responses of large yellow croaker (Larimichthys crocea) interleukin-8 and its receptor

机译：大黄鱼（Larimichthys Crocea）白细胞介素-8及其受体的功能识别和表达反应

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摘要

Interleukin-8 (IL-8 or chemokine (C-X-C motif) ligand 8, CXCL8) is a chemokine produced by multiple cell types. It promotes chemotaxis and phagocytosis via interaction with chemokine receptors CXCR1 and CXCR2. Using published data, IL-8 gene (LcIL-8) of the large yellow croaker (Larimichthys crocea) was cloned into the pcDNA3.1 plasmid, and an interleukin-8 receptor (LcCXCR2) was cloned into the pEGFP-N1 plasmid. Secratory expression of LcIL-8 in HEK293T cells was carried out, and product in culture medium was collected for LcCXCR2 stimulation in HEK293 cells. Following receptor internalization observation and intracellular signaling detection, the functional interaction of LcIL-8 and LcCXCR2 was further determined and the ERK phosphorylation signal activation mediated by LcCXCR2 was demonstrated. Quantitative real-time PCR analysis was used to analyze transcription level regulation of LcIL-8 and LcCXCR2 in various tissues of large yellow croaker. Expression of LcIL-8 and LcCXCR2 was elevated in the spleen, head kidney, and liver after Vibrio parahemolyticus challenge. Results illustrated the functional interaction between LcIL-8 and LcCXCR2 in mediating intracellular ERK1/2 phosphorylation signaling and suggested that the LcIL-8 and LcCXCR2 system is part of the immune response induced by V. Parahemolyticus in L. crocea.
机译：白细胞介素-8（IL-8或趋化因子（C-X-C motif）配体8，CXCL8）是由多个细胞类型产生的趋化因子。它通过与趋化因子受体CXCR1和CXCR2的相互作用促进趋化性和吞噬作用。使用公开的数据，大黄色克罗克（Larimichthys Crocea）的IL-8基因（Lcil-8）被克隆到PCDNA3.1质粒中，并且将白细胞介素-8受体（LCCXCR2）克隆到PEGFP-N1质粒中。进行了HEK293T细胞中LIC-8细胞的分泌表达，并在HEK293细胞中收集培养基中的产物。在受体内化观察和细胞内信号传导检测之后，进一步确定了LIC-8和LCCXCR2的功能性相互作用，并证明了由LCCXCR2介导的ERK磷酸化信号激活。定量实时PCR分析用于分析大黄鱼克罗克山脉各种组织中LIC-8和LCCXCR2的转录水平调节。在帕希莫溶血溶解后，脾脏，头肾和肝脏的表达升高。结果说明了LIC-8和LCCXCR2在介导细胞内ERK1 / 2磷酸化信号中的功能相互作用，并表明LIC-8和LCCXCR2系统是在L.番茄中的V.副磷酸盐溶解的免疫应答的一部分。

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  • 来源
    《Fish & Shellfish Immunology》 |2019年第2019期|共8页
  • 作者

    Wang Tianming; Liang Jing; Xiang Xiaowei; Yuan Jiajie; Chen Xu; Xiang Xingwei; Yang Jingwen;

  • 作者单位

    Zhejiang Ocean Univ Coll Marine Sci Natl Engn Res Ctr Marine Facil Aquaculture Zhoushan 316022 Zhejiang Peoples R China;

    Zhejiang Ocean Univ Coll Marine Sci Natl Engn Res Ctr Marine Facil Aquaculture Zhoushan 316022 Zhejiang Peoples R China;

    Zhejiang Ocean Univ Coll Marine Sci Natl Engn Res Ctr Marine Facil Aquaculture Zhoushan 316022 Zhejiang Peoples R China;

    Shaoxing Entry Exit Inspect &

    Quarantine Bur Comprehens Technol Serv Ctr Shaoxing 312000 Zhejiang Peoples R China;

    Zhejiang Ocean Univ Coll Marine Sci Natl Engn Res Ctr Marine Facil Aquaculture Zhoushan 316022 Zhejiang Peoples R China;

    Zhejiang Marine Dev Res Inst Tiyu Rd 10 Zhoushan 316000 Zhejiang Peoples R China;

    Zhejiang Ocean Univ Coll Marine Sci Natl Engn Res Ctr Marine Facil Aquaculture Zhoushan 316022 Zhejiang Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 水产、渔业;
  • 关键词

    Larimichthys crocea; IL-8; CXCR2; Functional interaction; Vibrio Parahemolyticus; immune response;

    机译：Larimichthys Crocea;IL-8;CXCR2;功能性相互作用;血管杀菌剂;免疫应答;

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