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首页> 外文期刊>Fish & Shellfish Immunology >Protection against Vibrio alginolyticus in pearl gentian grouper (female Epinephelus fuscoguttatus x male Epinephelus lanceolatu) immunized with an acfA-deletion live attenuated vaccine
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Protection against Vibrio alginolyticus in pearl gentian grouper (female Epinephelus fuscoguttatus x male Epinephelus lanceolatu) immunized with an acfA-deletion live attenuated vaccine

机译：用ACFA缺失活病毒疫苗免疫接种珍珠龙胆石斑鱼（雌性肾盂蛋白Fuscoguttatus X男性Epinephelus Lanceolatu）防止Vibrio Alginolyticus

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Vibrio alginolyticus is well-known as an opportunistic Grain-negative pathogen, which endangers the development of global aquaculture as well as human health. In this study, a Delta acfA mutant strain and complementation of the Delta acfA mutant (C-acfA) were constructed. The Delta acfA mutant was tested in pearl gentian grouper (female Epinephelus fuscoguttatus x male Epinephelus lanceolatu) to observe the changes in virulence and evaluate its potential as an attenuated live vaccine. The results showed that the Delta acfA mutant caused a high antibody titer and a significant reduction in the ability to colonize the intestine of pearl gentian grouper. Grouper vaccinated with Delta acfA mutant were more tolerant of the infection by virulent V. alginolyticus HY9901 without inducing clinical symptoms and obvious pathological changes. The relative percent survival value of pearl gentian grouper vaccinated with Delta acfA mutant intraperitoneal injection reached 81.1% after challenging with V. alginolyticus HY9901. The specific antibody titers immunized with Delta acfA was significantly higher than that in the PBS group. The antibody titer of Delta acfA group displayed the tendency of rising up from the first to fourth week and declining from fifth to eighth week and reached the peak at the fourth week. In the meanwhile, the expression level of genes associated with immunity, including IL-1 beta, TNF-alpha, IL-16, IgM, CD8 alpha and MHC-I alpha, was up-regulated after vaccination, indicating that the Delta acfA can induce effective and durable immune response in pearl gentian grouper and it may be an effective attenuated live vaccine candidate for the prevention of infections by V. alginolyticus.
机译：vibrio alginolyticus是众所周知的机会主义的谷物阴性病原体，危及全球水产养殖以及人类健康的发展。在该研究中，构建了δAcFA突变体菌株和δAcFA突变体（C-ACFA）的互补。 Delta ACFA突变体在珍珠龙胆格鲁珀（雌性Epinephelus fuscoguttatus X雄性Epinephelus lanceolatu）中进行了测试，以观察毒力的变化，并评估其作为减毒活疫苗的潜力。结果表明，Delta ACFA突变体引起高抗体滴度和显着降低了珍珠龙胆石斑鱼的肠道的能力。用Delta ACFA突变体接种的格鲁珀更耐受毒力V.Alginolyticus Hy9901的感染，而不会诱导临床症状和明显的病理变化。在与V.AlginolyTicus Hy9901具有挑战性后，珍珠冠脉冲疫苗接种的珍珠龙胆突变部门腹膜注射率的相对百分比达到81.1％。用Delta ACFA免疫的特异性抗体滴度明显高于PBS组中的抗体滴度。 Delta ACFA集团的抗体滴度显示出从第一至第四周起到升起的趋势，并从第五周下降到第四周，并在第四周达到峰值。同时，疫苗接种后上调了与免疫相关的基因的表达水平，包括IL-1β，TNF-α，IL-16，IgM，CD8α和MHC-1α，表明Delta ACFA可以在珍珠龙莲石斑鱼中诱导有效和耐用的免疫应答，可能是V.Alginolyticus预防感染的有效减毒的活疫苗候选者。

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