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首页> 外文期刊>Fish & Shellfish Immunology >A novel CC chemokine ligand 2 like gene from ayu Plecoglossus altivelis is involved in the innate immune response against to Vibrio anguillarum
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A novel CC chemokine ligand 2 like gene from ayu Plecoglossus altivelis is involved in the innate immune response against to Vibrio anguillarum

机译：来自Ayu Plecoglossus Altivelis的新型CC趋化因子配体2，如来自Ayu Plecoglossus Altivelis的基因，参与了对抗vibrio anguillarum的先天免疫反应

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Chemokine (C-C motif) ligand 2 (CCL2), also known as monocyte chemoattractant protein 1 (MCP-1), is one of the key chemokines that regulate migration and infiltration of monocytes/macrophages (MO/M Phi) in mammals. However, the functional repertoire of fish CCL2 remains unclear. Here, we identified a cDNA sequence encoding' a novel CCL2-like protein (PaCCL2L) in ayu, Plecoglossus altivelis. Sequence analysis revealed that PaCCL2L grouped with CCL2 homologs, and is most closely related to Mexican tetra (Astyanax mexicanus) and zebrafish (Danio rerio) homologs. PaCCL2 transcripts were expressed in all tested tissues from healthy ayu, with the highest level in the spleen. Upon Vibrio anguillarum infection, PaCCL2L transcripts increased significantly in tested tissues, including the liver, spleen, and head kidney. We then produced the recombinant PaCCL2L mature peptide (rPaCCL2L) by prokaryotic expression and generated the corresponding antibodies (anti-PaCCL2L). A significant increase in PaCCL2L protein and mRNA expression was observed in ayu MO/Me. following V. anguillarum challenge. Intraperitoneal injection of rPaCCL2L resulted in significantly improved survival and reduced tissue bacterial load in V. anguillarum-infected ayu. rPaCCL2L had a positive effect on the chemotaxis of MO/McD and neutrophils both in vitro and in vivo. Meanwhile, rPaCCL2L exhibited a positive effect on the chemotaxis of LPS-stimulated MO/M Phi (Ml type) in vitro, whereas it exhibited no chemotaxis effect on cAMP-stimulated MO/M Phi (M2 type). In addition, rPaCCL2L treatment exhibited an enhanced effect on MO/M Phi phagocytosis, bacterial killing, respiratory burst, and mRNA expression of proinflammatory cytokines, whereas anti-PaCCL2L treatment had an inhibitory effect. Our study demonstrates that PaCCL2L might play a role in the immune response of ayu against V. anguillarum infection through chemotactic recruitment and activation of MO/M Phi.
机译：趋化因子（C-C图谱）配体2（CCL2），也称为单核细胞化学蛋白1（MCP-1），是调节哺乳动物中单核细胞/巨噬细胞（MO / M PHI）的迁移和浸润的关键趋化因子之一。然而，鱼CCL2的功能性曲目仍然不清楚。在这里，我们鉴定了一种CDNA序列编码“新型CCL2样蛋白（PACCL2L）在AYU，Plecoglossus Altivelis。序列分析显示，与CCl2同源物中的PacCl2L与墨西哥Tetra（Astyanax Mexicanus）和斑马鱼（Danio Rerio）同源物最密切相关。 PacCl2转录物在来自健康AYU的所有测试组织中表达，脾脏中最高水平。在Vibrio Anguillarum感染后，PacCl2L转录物在测试组织中显着增加，包括肝脏，脾脏和头肾。然后我们通过原核表达制备重组PacCl 2L成熟肽（RPACCL 2L）并产生相应的抗体（抗-POL2L）。在Ayu Mo / Me中观察到PacCl2L蛋白和mRNA表达的显着增加。遵循V. Anguillarum挑战。腹膜内注射RPACCL2L导致V.Anuillarum-Cerved Ayu中的存活和减少组织细菌载荷显着提高。 RPACCL2L对体外和体内的MO / MCD和中性粒细胞的趋化性作用阳性作用。同时，RPACCL2L对体外LPS刺激的MO / M PHI（ML型）的趋化性表现出阳性作用，而在营养刺激的MO / M PHI（M2型）上表现出趋化性效应。此外，RPACCL2L治疗表现出对MO / M PHI吞噬作用，细菌杀伤，呼吸爆发和促胰岛细胞因子的mRNA表达的影响，而抗PACCL2L治疗具有抑制作用。我们的研究表明，PacCl2L可以通过趋化招募和激活Mo / M Phi激活Ayu对抗V.Anuillarum感染的影响。

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