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首页> 外文期刊>Fish & Shellfish Immunology >Cloning of three heat shock protein genes (HSP70, HSP90 alpha and HSP90 beta) and their expressions in response to thermal stress in loach (Misgurnus anguillicaudatus) fed with different levels of vitamin C
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Cloning of three heat shock protein genes (HSP70, HSP90 alpha and HSP90 beta) and their expressions in response to thermal stress in loach (Misgurnus anguillicaudatus) fed with different levels of vitamin C

机译：克隆三种热休克蛋白基因（HSP70，HSP90α和HSP90β）及其表达响应于饲喂不同水平的维生素C水平的泥鳅（Misgurnus Anguillicatuatus）的热应力

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Heat shock protein 70 (HSP70) and 90 (HSP90) are the most broadly studied proteins in HSP families. They play key roles in cells as molecular chaperones, in response to stress conditions such as thermal stress. In this study, full-length cDNA sequences of HSP70, HSP90 alpha and HSP90 beta from loath Misgurnus anguillicaudatus were cloned. The full-length cDNA of HSP70 in loath was 2332bp encoding 644 amino acids, while HSP90 alpha and HSP90 beta were 2586bp and 2678bp in length, encoding 729 and 727 amino acids, respectively. The deduced amino acid sequences of HSP70 in loath shared the highest identity with those of Megalobrama amblycephala and Cyprinus carpio. The deduced amino acid sequences of HSP90 alpha and HSP90 beta in loath both shared the highest identity with those of M. amblycephala. Their mRNA tissue expression results showed that the maximum expressions of HSP70, HSP90 alpha and HSP90 beta were respectively present in the intestine, brain and kidney of loath. Quantitative real-time PCR was employed to analyze the temporal expressions of HSP70, HSP90 alpha and HSP90 beta in livers of loaches fed with different levels of vitamin C under thermal stress. Expression levels of the three HSP genes in loath fed the diet without vitamin C supplemented at 0 h of thermal stress were significantly lower than those at 2 h, 6 h, 12 h and 24 h of thermal stress. It indicated that expressions of the three HSP genes were sensitive to thermal stress in loath. The three HSP genes in loaches fed with 1000 mg/kg vitamin C expressed significantly lower than other vitamin C groups at many time points of thermal stress, suggesting 1000 mg/kg dietary vitamin C might decrease the body damages caused by the thermal stress. This study will be of value for further studies into thermal stress tolerance in loath. (C) 2017 Elsevier Ltd. All rights reserved.
机译：热休克蛋白70（HSP70）和90（HSP90）是HSP家族中最广泛研究的蛋白质。它们在细胞中扮演分子伴侣的关键作用，响应于诸如热应力的应力条件。在该研究中，克隆了HSP70，HSP90α和Hsp90β的全长cDNA序列来自缠绕的Misgurnus anguillicatuatus。 HSP70的HSP70的全长cDNA为编码644个氨基酸的2332bp，而HSP90α和HSP90β的长度为2586bp和2678bp，分别编码729和727氨基酸。 Loath中HSP70的推导氨基酸序列与MegAlobrama Amblycephala和Cyprinus carpio的最高身份分享了最高的身份。 HSP90α和HSP90β的推导氨基酸序列在滞后两者都与M. Amblycephala的最高身份共享。它们的mRNA组织表达结果表明，HSP70，HSP90α和HSP90β的最大表达分别存在于肠道，脑和肾脏的肠道中。使用定量实时PCR分析HSP70，HSP90α和HSP90β在热应力下不同水平的维生素C喂养的肝脏中的HSP70，HSP90α和HSP90β的时间表达。在没有维生素C的恶臭中喂食饮食中的三个HSP基因的表达水平显着低于热应力的2小时，6小时，12小时和24小时。它表明，三个HSP基因的表达对恶性的热应力敏感。饲喂1000mg / kg维生素C的含量中的三个HSP基因显着低于其他维生素C组在许多时间的热应力点，表明1000mg / kg膳食维生素C可能会降低由热应力引起的身体损坏。本研究将具有进一步研究的价值，进一步研究了恶臭的热应力耐受性。 （c）2017 Elsevier Ltd.保留所有权利。

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