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首页> 外文期刊>Biochimica et Biophysica Acta. Protein Structure and Molecular Enzymology >Agrobacterium tumefaciens β-glucosidase is also an effective β-xylosidase, and has a high transglycosylation activity in the presence of alcohols
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Agrobacterium tumefaciens β-glucosidase is also an effective β-xylosidase, and has a high transglycosylation activity in the presence of alcohols

机译：根癌农杆菌β-葡糖苷酶也是有效的β-木糖苷酶，并且在醇存在下具有高的转糖基化活性。

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摘要

Agrobacterium tumefaciens β-glucosidase, Cbg1 was extensively characterised and found to be a retaining aryl-glucosidase and an aryl-xylosidase. Cbg1s specificity for p-nitrophenyl β-D-xylopyranoside was 73% that for p-nitrophenyl β-D-glucopyranoside when measured by the ratio k_(cat)/K_m. The enzyme also hydrolysed p-nitrophenyl β-D-fucopyranoside, and p-nitrophenyl β-D-galactopyranoside with moderate efficiency. The enzyme released only terminal glucose from p-nitrophenyl β-cellobioside and had a 20 000-fold preference for its natural substrate coniferin over cellobiose as indicated by the ratio k_(cat)/K_m. The enzyme was activated in the presence of 20 mM 1-propanol, 1-butanol, 1-pentanol, 1-hexanol, 1-heptanol, and 1-octanol. In the case of butanol this activation was investigated and shown to be due to transglycosylation activity with over 80% of p-nitrophenyl β-D-glucopyranoside being converted to 1-butyl β-D-glucopyranoside in the presence of Cbg1 and 100 mM 1-butanol.
机译：根癌农杆菌β-葡糖苷酶，Cbg1被广泛表征，发现是保留的芳基葡糖苷酶和芳基木糖苷酶。当用比率k_（cat）/ K_m测量时，对-硝基苯基β-D-吡喃吡喃糖苷的Cbg1s特异性为对-硝基苯基β-D-吡喃吡喃糖苷的Cbg1s特异性。该酶还以中等效率水解了对硝基苯基β-D-呋喃果糖苷和对硝基苯基β-D-吡喃半乳糖苷。该酶仅从对硝基苯基β-纤维二糖苷释放末端葡萄糖，并且其天然底物松柏油蛋白比纤维二糖具有2万倍的偏好，如比率k_（cat）/ K_m所示。在20 mM 1-丙醇，1-丁醇，1-戊醇，1-己醇，1-庚醇和1-辛醇的存在下激活酶。在丁醇的情况下，对该活化进行了研究，结果表明这是由于糖基转移活性引起的，在Cbg1和100 mM 1存在下，超过80％的对硝基苯基β-D-吡喃葡萄糖苷被转化为1-丁基β-D-吡喃葡萄糖苷。 -丁醇。

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