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首页> 外文期刊>Fish Physiology and Biochemistry >RNA-seq and microRNA-seq analysis of Japanese flounder (Paralichthys olivaceus) larvae treated by thyroid hormones
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RNA-seq and microRNA-seq analysis of Japanese flounder (Paralichthys olivaceus) larvae treated by thyroid hormones

机译：通过甲状腺激素治疗的日本比目鱼（Paralichthys Olivaceus）幼虫的RNA-SEQ和MicroRNA-SEQ分析

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摘要

Flatfish pigmentation is a complex process, affected by environmental factors including light, nutrients, and hormones. Of those, the thyroid hormone has been reported to increase the albinism rate of Japanese flounder (Paralichthys olivaceus). However, the underlying mechanism remains unclear. In the present study, triiodothyronine (T3), thyroxine, and thiourea were introduced into P. olivaceus larvae from 16 to 57 days after hatching (DAH). By comparison of albinism rate, T3 treatment and control larvae of 42 DAH were chosen for mRNA and miRNA high-throughput sequencing analyses. A total of 337 miRNAs were identified via miRNA-seq, and 12 miRNAs exhibited significantly differential expression patterns in D42_T3 versus D42_Con (TPM > 10, fold change >= 1.5 or = 0.67 and q = 0.05). These differentially expressed miRNAs targeted 3658 putative genes, which further enriched to 10 GO terms (q 0.05). RNA-seq identified 146 differentially expressed genes (DEGs) in D42_T3 versus D42_Con (vertical bar log(2) fold change vertical bar >1 and q 0.005), including pigmentation-related genes such as the receptor tyrosine-protein kinase erbB-3, pro-opiomelanocortin A, and melanotransferrin, and the growth-related gene somatotropin. These DEGs were significantly enriched to 15 GO terms and 8 KEGG pathways (q 0.05), which included several sugar metabolic pathways (glycolysis/gluconeogenesis and the pentose phosphate pathway). Integrated analysis revealed that 26 overlapping genes between DEGs and mRNAs were targeted by miRNAs. Furthermore, seven mRNA-miRNA pairs exhibited reversed regulation patterns. This provides important clues to understand the role of thyroid hormones in flatfish pigmentation.
机译：扁平鱼色素沉着是一种复杂的过程，受环境因素，包括光，营养素和激素。其中，据报道，甲状腺激素增加了日本比目鱼（Paralichthys Olivaceus）的复蛋白率。然而，潜在机制仍然不清楚。在本研究中，在孵化后16至57天内将三碘罗酮（T3），甲状腺素和硫脲引入P. Olivaceus幼虫中（DAH）。通过比较BRNA和MiRNA高通量测序分析选择42dAh的T3处理和控制幼虫。通过MiRNA-SEQ鉴定了337个miRNA，12 miRNA在D42_T3与D42_CON（TPM> 10中显示出显着的差异表达模式（TPM> 10，折叠变化> = 1.5或＆ = 0.05）。这些差异表达的miRNA靶向3658个诱导基因，其进一步富集至10种术语（Q＆ 0.05）。 RNA-SEQ鉴定D42_T3的差异表达基因（DEGS）与D42_CON（垂直条状（2）折叠垂直杆> 1和Q <0.005），包括色素沉着相关基因，例如受体酪氨酸 - 蛋白激酶erbB- 3，Pro-opiomelanocortina，和素蛋白转移素，和生长相关的基因躯体激素。这些可显着富集到15阶段和8 kegg途径（Q＆ 0.05），其包括几种糖代谢途径（糖酵解/葡糖生成和戊糖磷酸途径）。综合分析表明，在MiRNA靶向egs和mRNA之间的26个重叠基因。此外，七对mRNA-miRNA对表现出反向调节模式。这提供了了解甲状腺激素在扁平鱼色素沉着中的作用的重要线索。

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  • 来源
    《Fish Physiology and Biochemistry》 |2019年第4期|共12页
  • 作者

    Wang Na; Wang Renkai; Wang Ruoqing; Chen Songlin;

  • 作者单位

    Chinese Acad Fishery Sci Key Lab Sustainable Dev Marine Fisheries Minist Agr Yellow Sea Fisheries Res Inst 106 Nanjing Rd Qingdao 266071 Shandong Peoples R China;

    Chinese Acad Fishery Sci Key Lab Sustainable Dev Marine Fisheries Minist Agr Yellow Sea Fisheries Res Inst 106 Nanjing Rd Qingdao 266071 Shandong Peoples R China;

    Chinese Acad Fishery Sci Key Lab Sustainable Dev Marine Fisheries Minist Agr Yellow Sea Fisheries Res Inst 106 Nanjing Rd Qingdao 266071 Shandong Peoples R China;

    Chinese Acad Fishery Sci Key Lab Sustainable Dev Marine Fisheries Minist Agr Yellow Sea Fisheries Res Inst 106 Nanjing Rd Qingdao 266071 Shandong Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 水产、渔业;
  • 关键词

    Japanese flounder (Paralichthys olivaceus); Albinism; Thyroid hormone; Glycolysis;

    机译：日本比目鱼（Paralichthys Olivaceus）;复合主义;甲状腺激素;糖醇分析;

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