...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Fish Physiology and Biochemistry >Cloning, molecular characterization, and tissue differential expression of connective tissue growth factor (ctgf) of grass carp
【24h】

Cloning, molecular characterization, and tissue differential expression of connective tissue growth factor (ctgf) of grass carp

机译：草鲤鱼结缔组织生长因子（CTGF）的克隆，分子表征和组织差异表达

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Connective tissue growth factor (ctgf) is involved in the proliferation, migration, adhesion of cell, and the constituent of extracellular matrix, which plays an important role in embryogenesis, angiogenesis, wound repair, and fibrosis diseases. In this study, the cDNA sequence of grass carp ctgf gene was cloned by rapid amplification of cDNA ends (RACE) method; then, the characteristics of this gene and the predicted protein sequence were analyzed by bioinformatics methods, and the tissue differential expression pattern was detected by the quantitative real-time PCR. The results showed that the grass carp ctgf gene has a full-length of 2223bp, encoding 343 amino acids. The deduced CTGF protein is a hydrophilic and secretary protein with a molecular mass of 37,978.2Da and an isoelectric point of 8.22. The signal peptide locates between residue positions 1 and 22 of the polypeptide chain. The protein contains alpha-helix, beta-strand, and loops. The CTGF protein of grass carp shows a homology of 98%, 96%, 91%, and 91% with Wuchang bream (Megalobrama amblycephala), zebrafish (Danio rerio), common carp (Cyprinus carpio), and Mexican tetra (Astyanax mexicanus). The grass carp ctgf gene expressed significantly higher in blood and spleen than that in other tissues (P 0.05). The low expression tissues included the heart, gill, skin, muscle, kidney, brain, and intestinal, and the lowest expression tissue was the liver. The results are consistent with the function of this gene.
机译：结缔组织生长因子（CTGF）涉及细胞的增殖，迁移，粘附性，细胞外基质的组分，在胚胎发生，血管生成，伤口修复和纤维化疾病中起着重要作用。在该研究中，通过快速扩增cDNA末端（种族）方法来克隆草鲤CTGF基因的cDNA序列;然后，通过生物信息学方法分析该基因的特征和预测的蛋白质序列，通过定量实时PCR检测组织差异表达模式。结果表明，草鲤CTGF基因具有全长2223bp，编码343氨基酸。所推出的CTGF蛋白是亲水和秘书蛋白质，其分子量为37,978.2Da和等电点为8.22。信号肽位于多肽链的残基位置1和22之间。蛋白质含有α-螺旋，β-股和环。草鲤鱼的CTGF蛋白显示出98％，96％，91％和91％的同源性，武昌鲷（Megalobrama Amblycephala），斑马鱼（Danio Rerio），鲤鱼（Cyprinus carpio）和墨西哥Tetra（astyanax mexicanus） 。草鲤CTGF基因在血液和脾脏显着高于其他组织（P <0.05）。低表达组织包括心脏，鳃，皮肤，肌肉，肾，脑和肠道，最低表达组织是肝脏。结果与该基因的功能一致。

著录项

  • 来源
    《Fish Physiology and Biochemistry》 |2019年第4期|共13页
  • 作者

    Pan Wen-Qian; Wang Jun-Peng; Tu Zhi-Han; Gan Tian; Hu Jing; Wei Jing; Leng Xiang-Jun; Li Xiao-Qin;

  • 作者单位

    Shanghai Ocean Univ Coll Fisheries &

    Life Sci 999 Huchenghuan Rd Shanghai 201306 Peoples R China;

    Shanghai Ocean Univ Coll Fisheries &

    Life Sci 999 Huchenghuan Rd Shanghai 201306 Peoples R China;

    Shanghai Ocean Univ Coll Fisheries &

    Life Sci 999 Huchenghuan Rd Shanghai 201306 Peoples R China;

    Shanghai Ocean Univ Coll Fisheries &

    Life Sci 999 Huchenghuan Rd Shanghai 201306 Peoples R China;

    Shanghai Ocean Univ Coll Fisheries &

    Life Sci 999 Huchenghuan Rd Shanghai 201306 Peoples R China;

    Shanghai Ocean Univ Coll Fisheries &

    Life Sci 999 Huchenghuan Rd Shanghai 201306 Peoples R China;

    Shanghai Ocean Univ Coll Fisheries &

    Life Sci 999 Huchenghuan Rd Shanghai 201306 Peoples R China;

    Shanghai Ocean Univ Coll Fisheries &

    Life Sci 999 Huchenghuan Rd Shanghai 201306 Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 水产、渔业;
  • 关键词

    ctgf; RACE; Tissue differential expression; Gene clone; Grass carp;

    机译：CTGF;种族;组织差异表达;基因克隆;草鲤鱼;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号