The use of multiwavelength kinetic analysis approach to identify and characterize intermediate complexes in the reductive amination reaction catalyzed by bovine liver glutamate dehydrogenase

Swapan K. Saha; Steven J. Maniscalco; Harvey F. Fisher

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The use of multiwavelength kinetic analysis approach to identify and characterize intermediate complexes in the reductive amination reaction catalyzed by bovine liver glutamate dehydrogenase

机译：使用多波长动力学分析方法鉴定和表征牛肝谷氨酸脱氢酶催化的还原胺化反应中的中间配合物

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摘要

A multiwavelength transient-state kinetic study of the glutamate dehydrogenase catalyzed reaction has proven that an α-iminoglutarate complex is an observable intermediate in the reverse direction. It also shows the existence of two enzyme-NADPH-ketoglutarate complexes, only one of which reacts with ammonia rapidly.

机译：谷氨酸脱氢酶催化反应的多波长瞬态动力学研究已证明，α-亚氨基戊二酸酯络合物是可观察到的反向中间体。它还显示了两种酶-NADPH-酮戊二酸复合物的存在，其中只有一种与氨快速反应。

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《Biochimica et Biophysica Acta. Protein Structure and Molecular Enzymology》 |1998年第0期|共5页
作者
Swapan K. Saha; Steven J. Maniscalco; Harvey F. Fisher;
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原文格式 PDF
正文语种 eng
中图分类生物化学;分子生物学;
关键词
enzyme; dehydrogenase; transient state kinetics;

机译：酶;脱氢酶;瞬态动力学;

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1. The use of multiwavelength kinetic analysis approach to identify and characterize intermediate complexes in the reductive amination reaction catalyzed by bovine liver glutamate dehydrogenase [J] . Swapan K. Saha, Steven J. Maniscalco, Harvey F. Fisher Biochimica et Biophysica Acta. Protein Structure and Molecular Enzymology . 1998,第0期

机译：使用多波长动力学分析方法鉴定和表征牛肝谷氨酸脱氢酶催化的还原胺化反应中的中间配合物
2. Identification and characterization of kinetically competent carbinolamine and alpha-iminoglutarate complexes in the glutamate dehydrogenase-catalyzed oxidation of L-glutamate using a multiwavelength transient state approach [J] . Maniscalco SJ., Fisher HF., Saha SK. Biochemistry . 1998,第41期

机译：使用多波长瞬态方法鉴定和表征谷氨酸脱氢酶催化的L-谷氨酸氧化反应中具有动力学活性的甲醇胺和α-亚氨基戊二酸酯复合物
3. The location of active site opening and closing events in the prehydride transfer phase of the oxidative deamination reaction catalyzed by bovine liver glutamate dehydrogenase using a novel pH jump approach. [J] . Saha SK, Fisher HF Biochimica et biophysica acta: international journal of biochemistry and biophysics . 1999,第1期

机译：使用新型pH跳跃方法在牛肝谷氨酸脱氢酶催化的氧化脱氨反应的预氢化物转移阶段中，活性位点打开和关闭事件的位置。
4. Ruthenium-Catalyzed Novel Reaction via Cumulenylidene Complexes as Key Intermediates [C] . Yoshihiro Yamauchi, Masahiro Yuki, Yoshihiro Miyake Symposium on Organometallic Chemistry . 2007

机译：钌催化的新型反应通过水蓝基络合物作为关键中间体
5. Palladium-catalyzed reductive cross-coupling reactions and the analysis of site-selectivity in rhodium-catalyzed C-H amination reactions. [D] . DeLuca, Ryan James. 2014

机译：钯催化的还原交叉偶联反应和铑催化的C-H胺化反应中的位点选择性分析。
6. Kinetic studies of glutamate dehydrogenase. The reductive amination of 2-oxoglutarate [O] . P. C. Engel, K. Dalziel 1970

机译：谷氨酸脱氢酶的动力学研究。 2-氧戊二酸酯的还原胺化
7. Kinetic studies of glutamate dehydrogenase. The reductive amination of 2-oxoglutarate [O] . Engel, P. C., Dalziel, K. 1970

机译：谷氨酸脱氢酶的动力学研究。 2-氧戊二酸酯的还原胺化
8. Kinetic studies of dogfish liver glutamate dehydrogenase with diphosphopyridine nucleotide and the effect of added salts [R] . Corman, L., Kaplan, N. O. 1967

机译：用二磷酸吡啶核苷酸对鲨鱼肝谷氨酸脱氢酶的动力学研究及加盐的影响

The use of multiwavelength kinetic analysis approach to identify and characterize intermediate complexes in the reductive amination reaction catalyzed by bovine liver glutamate dehydrogenase

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