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首页> 外文期刊>Fungal Genetics and Biology >Complex epigenetic regulation of alkaloid biosynthesis and host interaction by heterochromatin protein I in a fungal endophyte-plant symbiosis
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Complex epigenetic regulation of alkaloid biosynthesis and host interaction by heterochromatin protein I in a fungal endophyte-plant symbiosis

机译：非致铬素蛋白I在真菌内蛋白酶共生中的生物碱生物合成和宿主相互作用的复杂表观遗传调节

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摘要

Epichloe festucae forms mutualistic symbiotic interactions with grasses of the Loliurn and Festuca genera. Protection from insect and mammalian herbivory are the best-documented host benefits of these associations. The two main classes of anti-mammalian alkaloids synthesized by E. festucae are the ergot alkaloids and indole diterpenes, of which ergovaline and lolitrems are the principal terminal products. Synthesis of both metabolites require multiple gene products encoded by clusters of 11 genes located at the subtelomeric regions of chromosomes I and III respectively. These loci are essentially unexpressed in axenic culture but among the most highly expressed genes in planta. We show here that heterochromatin 1 protein (HepA) is an important component of the regulatory machinery that maintains these loci in a silent state in culture. Deletion of this gene led to derepression of eas and km gene expression under non-symbiotic culture conditions. Although there was no obvious culture phenotype, RNAseq analysis revealed that around 1000 genes were differentially expressed in the Delta hepA mutant compared to wild type with just one-third upregulated. Inoculation of the Delta hepA mutants into seedlings of Lolitun perenne led to a severe host interaction phenotype characterized by a reduction in tiller length but an increase in tiller number. Hyphae within the leaves of these associations were much more abundant in the intercellular spaces of the leaves and aberrantly colonized the vascular bundles. This physiological change was accompanied by a dramatic change in the transcriptome with around 900 genes differentially expressed, with two thirds of these upregulated. This major physiological change was accompanied by a decrease in ltm gene expression and loss of the ability to synthesize lolitrems. These results show that HepA has an important role in controlling the chromatin state of these sub-telomeric secondary metabolite genes, including their symbiosis-specific regulation.
机译：Epichloe Festurcae与Loliurn和Festuca属的草形成互动的共生互动。保护昆虫和哺乳动物草食病是这些协会的最优秀的主机福利。由E. Festucape合成的两种主要类抗哺乳动物生物碱是麦角生物碱和吲哚二萜，其中ergovaline和Lolitems是主要终端产物。两种代谢物的合成需要分别由位于染色体I和III染色体细制区域的11个基因簇编码的多个基因产物。这些基因座在腋生中基本上是不表达的，但在植物中最高表达的基因中。我们在这里展示了异铬胺1蛋白（HEPA）是监管机械的重要组成部分，其在培养的沉默状态下维持这些基因座。在非共生培养条件下，缺失该基因导致DEREPLACE的EAS和KM基因表达。虽然没有明显的培养表型，但是，与野生型相比，RNA淀粉分析表明，与野生型相比，在Delta HEPA突变体中差异地表达了大约1000个基因。将Delta HEPA突变体接种到Lolitun Perenne的幼苗中导致了严重的宿主相互作用表型，其特征在于分蘖长度，但耕作数量增加。在这些关联的叶片内的菌丝在叶子的细胞间空间中较大，并且使血管束的异常殖民。这种生理变化伴随着转录组的显着变化，大约900个基因差异表达，其中三分之二即将上调。这种主要的生理变化伴随着LTM基因表达的减少和丧失合成Lolitems的能力。这些结果表明，HEPA在控制这些分子次级次要代谢物基因的染色质状态方面具有重要作用，包括其共生特异性调节。

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  • 来源
    《Fungal Genetics and Biology》 |2019年第2019期|共13页
  • 作者

    Chujo Tetsuya; Lukito Yonathan; Eaton Carla J.; Dupont Pierre-Yves; Johnson Linda J.; Winter David; Cox Murray P.; Scott Barry;

  • 作者单位

    Massey Univ Sch Fundamental Sci Private Bag 11 222 Palmerston North 4442 New Zealand;

    Massey Univ Sch Fundamental Sci Private Bag 11 222 Palmerston North 4442 New Zealand;

    Massey Univ Sch Fundamental Sci Private Bag 11 222 Palmerston North 4442 New Zealand;

    Massey Univ Sch Fundamental Sci Private Bag 11 222 Palmerston North 4442 New Zealand;

    AgResearch Ltd Grasslands Res Ctr Palmerston North New Zealand;

    Massey Univ Sch Fundamental Sci Private Bag 11 222 Palmerston North 4442 New Zealand;

    Massey Univ Sch Fundamental Sci Private Bag 11 222 Palmerston North 4442 New Zealand;

    Massey Univ Sch Fundamental Sci Private Bag 11 222 Palmerston North 4442 New Zealand;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 Q939.5;
  • 关键词

    Epichloe; Endophyte; HP1; Symbiosis; Transcriptome;

    机译：Epichloe;内心;HP1;共生;转录组;

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