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首页> 外文期刊>Marine genomics >De novo transcriptome assembly and gene expression profile of thermally challenged green abalone (Haliotis fulgens: Gastropoda) under acute hypoxia and hypercapnia
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De novo transcriptome assembly and gene expression profile of thermally challenged green abalone (Haliotis fulgens: Gastropoda) under acute hypoxia and hypercapnia

机译：De Novo转录组组件和基因表达型在急性缺氧和Hypercapnia下的热攻击绿色鲍鱼（Haliotis Fulgens：Gastropoda）

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摘要

Transcriptional regulation constitutes a rapid response of marine organisms facing stressful environmental conditions, such as the concomitant exposure to warming, ocean acidification and hypoxia under climate change. In previous studies, we investigated whole-organism physiological patterns and cellular metabolism in gill and muscle of the marine gastropod Haliotis fulgens in response to increasing temperature (18 degrees C to 32 degrees C at +3 degrees C per day) under hypoxia (50% air saturation), hypercapnia (1000 mu atrn pCO(2)) and both factors combined. Here, we report investigations of the molecular responses of H. fulgens to temperature and identify mechanisms concomitantly affected by hypoxia and hypercapnia. A de novo transcriptome assembly with subsequent quantitative PCR and correlation network analysis of genes involved in the molecular response were used to unravel the correlations between gene expression patterns under the different experimental conditions. The correlation networks identified a shift from the expression of genes involved in energy metabolism (down-regulated) to the up-regulation of Hsp70 during warming under all experimental conditions in gill and muscle, indicating a strong up-regulation of damage prevention and repair systems at sustained cellular energy production. However, a higher capacity for anaerobic succinate production was evicted in gill, matching with observations from our previous studies indicating succinate accumulation in gill but not in muscle. Additionally, warming under hypoxia and hypercapnia kept mRNA levels of citrate synthase in both tissues unchanged following a similar pattern as muscle enzyme capacity from a previous study, suggesting an emphasis on maintaining rather than down-regulating mitochondrial activity.
机译：转录调节构成了面临压力环境条件面临的海洋生物的快速反应，例如在气候变化下伴随着热化，海洋酸化和缺氧。在先前的研究中，我们在缺氧下的温度（每天18℃至32摄氏度为+ 3℃，每天18℃至32℃）的鳃和肌肉的鳃和肌肉中的全生物体生理模式和细胞代谢。在缺氧下（50％）空气饱和度），Hypercapnia（1000 mu ATRN PCO（2））和两个因素合并。在此，我们报告了H. umpgens的分子反应的调查，并鉴定受缺氧和HyproCapnia伴随的伴随的机制。使用随后的定量PCR的DE Novo转录组件和涉及分子响应中涉及的基因的相关网络分析用于在不同的实验条件下解开基因表达模式之间的相关性。相关网络鉴定了从鳃和肌肉中的所有实验条件下的温暖期间涉及能量代谢（下调）的基因表达到Hsp70的上调的转变，表明损伤防治系统的强大上调在持续的细胞能量生产。然而，在鳃中驱逐吞食厌氧琥珀酸盐的产能，与我们以前的研究的观察结果相匹配，这表明鳃中的琥珀酸累积而不是肌肉。另外，在缺氧和Hypercapnia下升温，在与先前研究的肌肉酶产能相似的情况下，两种组织中的两种组织中的柠檬酸盐合酶的mRNA水平保持不变，表明重视维持而不是下调线粒体活性。

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  • 来源
    《Marine genomics》 |2019年第2019期|共9页
  • 作者

    Tripp-Valdez Miguel A.; Harms Lars; Poertner Hans O.; Teresa Sicard M.; Lucassen Magnus;

  • 作者单位

    Helmholtz Ctr Polar &

    Marine Res Integrat Ecophysiol Alfred Wegener Inst Handelshafen 12 D-27570 Bremerhaven Germany;

    Helmholtz Ctr Polar &

    Marine Res Integrat Ecophysiol Alfred Wegener Inst Handelshafen 12 D-27570 Bremerhaven Germany;

    Helmholtz Ctr Polar &

    Marine Res Integrat Ecophysiol Alfred Wegener Inst Handelshafen 12 D-27570 Bremerhaven Germany;

    Inst Politecn Nacl Ctr Invest Biol Noroeste SC CIBNOR La Paz 23096 Baja California Mexico;

    Helmholtz Ctr Polar &

    Marine Res Integrat Ecophysiol Alfred Wegener Inst Handelshafen 12 D-27570 Bremerhaven Germany;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 遗传学;
  • 关键词

    Cellular stress response; Climate change; Gastropod; Gene expression; qPCR;

    机译：细胞应激反应;气候变化;胃肠杆菌;基因表达;QPCR;

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