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首页> 外文期刊>Marine genomics >Muscle and liver transcriptome characterization and genetic marker discovery in the farmed meagre, Argyrosomus regius
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Muscle and liver transcriptome characterization and genetic marker discovery in the farmed meagre, Argyrosomus regius

机译：养殖微薄的肌肉和肝脏转录组特征和遗传标志发现，argyrosomus regius

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摘要

Meagre (Argyrosomus regius), a teleost fish of the family Sciaenidae, is part of a group of marine fish species considered new for Mediterranean aquaculture representing the larger fish cultured in the region. Meagre aquaculture started similar to 25 years ago in West Mediterranean, and the supply of juveniles has been dominated by few hatcheries. This fact has raised concerns on possible inbreeding, urging the need for genetic information on the species and for an assessment of the polymorphisms found in the genome. To that end we characterized the muscle and liver transcriptome of a pool of meagre individuals, from different families and phenotypic size, to obtain a backbone that can support future studies regarding physiology, immunology and genetics of the species. The assembled transcripts were assigned to a wide range of biological processes including growth, reproduction, metabolism, development, stress and behavior. Then, to infer its genetic diversity and provide a catalogue of markers for future use, we scanned the reconstructed transcripts for polymorphic genetic markers. Our search revealed a total of 42,933 high quality SNP and 20,581 STR markers. We found a relatively low rate of polymorphism in the transcriptome that may indicate that inbreeding has taken place. This study has led to a catalogue of genetic markers at the expressed part of the genome and has set the ground for understanding growth and other traits of interest in meagre.
机译：Meager（Argyrosomus Regius）是家庭Sciaenidae的紧象鱼，是一群海洋鱼类的一部分，被认为是地中海水产养殖的新型，代表该地区培养的较大鱼类。微薄的水产养殖开始与25年前在西地中海开始，少年供应是少量孵化场所主导。这一事实提出了对可能的近亲繁育的担忧，促进了对物种的遗传信息和对基因组中发现的多态性的需求。为此，我们以不同的家庭和表型尺寸的肌肉和肝脏转录组特征在于，来自不同的家庭和表型尺寸，以获得能够支持有关物种生理，免疫学和遗传学研究的未来研究的骨干。将组装的转录物分配给广泛的生物过程，包括生长，繁殖，代谢，发育，压力和行为。然后，要推断其遗传多样性并为未来使用提供标记的目录，我们扫描了用于多态遗传标记的重建转录物。我们的搜索总共揭示了42,933个高质量的SNP和20,581个STR标记。我们在转录组中发现了相对低的多态性速率，这可能表明已经发生了近亲繁殖。该研究导致了基因组的表达部分的遗传标志物目录，并为理解微薄的增长和其他感兴趣的特征设定了理解。

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  • 来源
    《Marine genomics》 |2018年第2018期|共6页
  • 作者

    Manousaki T.; Tsakogiannis A.; Lagnel J.; Kyriakis D.; Duncan N.; Estevez A.; Tsigenopoulos C. S.;

  • 作者单位

    Hellen Ctr Marine Res Inst Marine Biol Biotechnol &

    Aquaculture Iraklion Greece;

    Hellen Ctr Marine Res Inst Marine Biol Biotechnol &

    Aquaculture Iraklion Greece;

    INRA PACA UR GAFL 1052 67 Allee Chenes CS 60 094 F-84143 Montfavet France;

    Hellen Ctr Marine Res Inst Marine Biol Biotechnol &

    Aquaculture Iraklion Greece;

    IRTA Tarragona Spain;

    IRTA Tarragona Spain;

    Hellen Ctr Marine Res Inst Marine Biol Biotechnol &

    Aquaculture Iraklion Greece;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 遗传学;
  • 关键词

    Aquaculture; RNASeq; SNPs; STFts;

    机译：水产养殖;rnaseq;snps;stfts;

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