De novo transcriptome sequencing of an estuarine amphipod Grandidierella japonica exposed to zinc

Hiki Kyoshiro; Nakajima Nobuyoshi; Watanabe Haruna; Nakajima Fumiyuki; Tobino Tomohiro

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De novo transcriptome sequencing of an estuarine amphipod Grandidierella japonica exposed to zinc

机译：De Novo转录组测序的酯氨基Amphipod Grandidierella japonica暴露于锌

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Despite the ecological and ecotoxicological importance of the amphipod Grandidierella japonica, there is little molecular information on this species. We performed de novo transcriptome sequencing of G. japonica under zinc exposure to develop toxicant responsive transcriptome resources. Using the Ion Torrent platform we obtained 11 million raw reads, which were assembled into 105,166 transcripts (N50: 511 bases). The obtained sequences include many stress- and defence-related genes such as antioxidant genes and members of the cytochrome P450 family as well as potential reference genes. Our transcriptome data of G. japonica should be a useful reference for future ecological and ecotoxicological studies.

机译：尽管Amphipod Grandidierella japonica的生态和生态毒理学，但在这个物种上几乎没有分子信息。我们在锌暴露下进行了japonica的Novo转录组测序，以促进毒性响应转录组资源。使用离子Torrent平台，我们获得了1100万原料读数，组装成105,166转录物（N50：511碱基）。所获得的序列包括许多应激和防御相关基因，例如抗氧化基因和细胞色素P450家族的成员以及潜在的参考基因。我们的G. japonica的转录组数据应该是未来生态和生态毒理学研究的有用参考。

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来源
《Marine genomics》 |2018年第2018期|共4页
作者
Hiki Kyoshiro; Nakajima Nobuyoshi; Watanabe Haruna; Nakajima Fumiyuki; Tobino Tomohiro;
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作者单位

Univ Tokyo Dept Urban Engn Bunkyo Ku Hongo 7-3-1 Tokyo 1138656 Japan;

Natl Inst Environm Studies Ctr Environm Biol &

Ecosystem Studies Onogawa 16-2 Tsukuba Ibaraki 3058506 Japan;

Natl Inst Environm Studies Ctr Environm Risk Res Onogawa 16-2 Tsukuba Ibaraki 3058506 Japan;

Univ Tokyo Dept Urban Engn Bunkyo Ku Hongo 7-3-1 Tokyo 1138656 Japan;

Univ Tokyo Environm Sci Ctr Bunkyo Ku Hongo 7-3-1 Tokyo 1130033 Japan;

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原文格式 PDF
正文语种 eng
中图分类遗传学;
关键词
De novo transcriptome sequencing; Grandidierella japonica; Zinc; Amphipod; Biomarker;

机译：de novo转录组测序;祖先japonica;zinc;amphipod;生物标志物;

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专利

1. De novo transcriptome sequencing of an estuarine amphipod Grandidierella japonica exposed to zinc [J] . Hiki Kyoshiro, Nakajima Nobuyoshi, Watanabe Haruna, Marine genomics . 2018,第期

机译：De Novo转录组测序的酯氨基Amphipod Grandidierella japonica暴露于锌
2. Bioavailability of zinc in the sediment to the estuarine amphipod Grandidierella japonica [J] . Jianbo Han, Deyi Ma, Xie Quan, Hydrobiologia . 2005,第1期

机译：沉积物中锌对河口两栖纲日本大鳄的生物利用度
3. De novo transcriptome assembly of the amphipod Gammarus chevreuxi exposed to chronic hypoxia [J] . Collins Michael, Tills Oliver, Spicer John I., Marine genomics . 2017,第期

机译：De Novo转录组组装的Amphipodγ曲氏曲霉暴露于慢性缺氧
4. De Novo Transcriptome Sequencing of a Benthic Ostracod Heterocypris Incongruens Exposed to Zinc [C] . Masanon TSUCHIKAWA, Kyoshiro HIKI, Fumiyuki NAKAJIMA, Water and environment technology conference . 2018

机译：暴露于锌的底栖鸵鸟异质杂种的从头转录组测序。
5. Assessment of biochemical, developmental, and reproductive responses in meiobenthic copepods and estuarine amphipods exposed to potential endocrine-modulating chemicals. [D] . Block, David Steven. 2006

机译：评估暴露于潜在内分泌调节化学物质的中足底ben足动物和河口两栖动物的生化，发育和生殖反应。
6. De Novo Sequencing-Based Transcriptome and Digital Gene Expression Analysis Reveals Insecticide Resistance-Relevant Genes in Propylaea japonica (Thunberg) (Coleoptea: Coccinellidae) [O] . Liang-De Tang, Xing-Min Wang, Feng-Liang Jin, -1

机译：基于De Novo测序的转录组和数字基因表达分析揭示了日本瓢虫（Thunberg）中与杀虫剂抗性相关的基因（鞘翅目：瓢虫科）
7. De novo sequencing-based transcriptome and digital gene expression analysis reveals insecticide resistance-relevant genes in Propylaea japonica (Thunberg) (Coleoptea: Coccinellidae). [O] . Liang-De Tang, Xing-Min Wang, Feng-Liang Jin, 2014

机译：基于从头测序的转录组和数字基因表达分析揭示了日本propylaea japonica（Thunberg）（Coleoptea：Coccinellidae）中的杀虫剂抗性相关基因。

De novo transcriptome sequencing of an estuarine amphipod Grandidierella japonica exposed to zinc

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