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首页> 外文期刊>Marine genomics >An important resource for understanding bio-adhesion mechanisms: Cement gland transcriptomes of two goose barnacles, Pollicipes pollicipes and Lepas anatifera (Cirripedia, Thoracica)
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An important resource for understanding bio-adhesion mechanisms: Cement gland transcriptomes of two goose barnacles, Pollicipes pollicipes and Lepas anatifera (Cirripedia, Thoracica)

机译：理解生物粘附机制的重要资源：两只鹅藤壶的水泥腺转录om，Pollicipes Pollicipes和Lepas Anatifera（Cirripedia，Thoracica）

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摘要

Barnacles are sessile organisms globally distributed in marine and estuarine environments. The production of protein-based adhesives by cement glands is crucial for these organisms and plays a vital role in substrate attachment, thus inspiring biotechnological applications. Moreover, barnacles belong to bio-fouling communities, whose fixation onto man-made surfaces is of interest in terms of control and imposes an enormous economic cost on maritime industries. Here, we generated the first de novo assembly of cement gland transcriptomes of Pollicipes pollicipes (rocky-shore goose barnacle) and Lepas anatifera (pelagic goose barnacle) (Cirripedia, Thoracica), to generate molecular resources for understanding the bio-adhesion process in Cirripedia and to provide data for biotechnology. Via the Illumina next-generation sequencing technology, a total of 43.7 and 40.2 million (M) paired-end (PE) clean reads were obtained for P. pollicipes (PP) and L. anatifera (LA), respectively. De novo assemblies resulted in 116,377 transcripts for PP and 94,143 for LA, with N50 s of 1181 and 1028 bp, respectively. The Benchmarking Universal Single-Copy Orthologs (BUSCO) showed 78.7% (PP) and 91.7% (LA) of transcriptome completeness, in terms of expected gene content, for the Arthropoda phylum in each species. These results represent a substantial resource for expanding the knowledge on Cirripedia adhesion mechanisms and for contributions to the gene annotation of Crustacea where molecular resources are presently insufficient.
机译：藤壶是在海洋和河口环境中全球分布的无柄生物。通过水泥腺体生产蛋白质的粘合剂对这些生物来说至关重要，并在基材附件中起着至关重要的作用，从而鼓舞生物技术应用。此外，藤壶属于生物污染社区，其固定在人造表面上对控制方面感兴趣，并对海洋工业产生巨大的经济成本。在这里，我们生成了Pollicipes Pollicipes（岩石脚鹅钻石）和Lepas Anatifera（Pelagic Goose Barnacle）（Cirripedia，Thoracica）的第一次De Novo组装，以产生用于了解Cirripedia中生物粘附过程的分子资源并提供生物技术数据。通过Illumina下一代测序技术，分别为P.Policipes（PP）和L.Anatifera（La）分别获得了43.7和40.2百万（M）配对末端（PE）清洁读数。 DE Novo组件分别导致LA和94,143的116,377转录物，分别为1181和1028bp的N50 S.基准通用单拷贝直系显示（Busco）显示了每种物种中的节肢动物含量的预期基因含量的78.7％（PP）和转录组完整性的91.7％（LA）。这些结果代表了扩大对肝硬化粘附机制的知识以及对甲壳成分的基因注释的贡献的重要资源，其中分子资源目前不足。

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  • 来源
    《Marine genomics》 |2019年第2019期|共5页
  • 作者

    Machado Andre M.; Sarropoulou Elena; Castro L. Filipe C.; Vasconcelos Vitor; Cunha Isabel;

  • 作者单位

    Univ Porto Interdisciplinary Ctr Marine &

    Environm Res CIIMA Ave Gen Norton de Matos S-N P-4450238 Terminal Cruzeiros Porto Matosinhos Portugal;

    Hellen Ctr Marine Res Inst Marine Biol Biotechnol &

    Aquaculture IMBBC Iraklion Greece;

    Univ Porto Interdisciplinary Ctr Marine &

    Environm Res CIIMA Ave Gen Norton de Matos S-N P-4450238 Terminal Cruzeiros Porto Matosinhos Portugal;

    Univ Porto Interdisciplinary Ctr Marine &

    Environm Res CIIMA Ave Gen Norton de Matos S-N P-4450238 Terminal Cruzeiros Porto Matosinhos Portugal;

    Univ Porto Interdisciplinary Ctr Marine &

    Environm Res CIIMA Ave Gen Norton de Matos S-N P-4450238 Terminal Cruzeiros Porto Matosinhos Portugal;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 遗传学;
  • 关键词

    Barnacles; Bio-adhesion; Biofouling; Subaquatic glues; Biomimetic materials; Underwater adhesives;

    机译：藤壶;生物粘附;生物污染;胎面胶;仿生材料;水下粘合剂;

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