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首页> 外文期刊>Medicine and science in sports and exercise >One Week of Step Reduction Lowers Myofibrillar Protein Synthesis Rates in Young Men
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One Week of Step Reduction Lowers Myofibrillar Protein Synthesis Rates in Young Men

机译：一周的一周减少了年轻人的肌原纤维蛋白综合率

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Purpose Across the lifespan, physical activity levels decrease and time spent sedentary typically increases. However, little is known about the impact that these behavioral changes have on skeletal muscle mass regulation. The primary aim of this study was to use a step reduction model to determine the impact of reduced physical activity and increased sedentary time on daily myofibrillar protein synthesis rates in healthy young men. Methods Eleven men (22 +/- 2 yr) completed 7 d of habitual physical activity (HPA) followed by 7 d of step reduction (SR). Myofibrillar protein synthesis rates were determined during HPA and SR using the deuterated water ((H2O)-H-2) method combined with the collection of skeletal muscle biopsies and daily saliva samples. Gene expression of selected proteins related to muscle mass regulation and oxidative metabolism were determined via real time reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Results Daily step count was reduced by approximately 91% during SR (from 13,054 +/- 2763 steps per day to 1192 +/- 330 steps per day; P < 0.001) and this led to an increased contribution of sedentary time to daily activity (73% +/- 6% to 90% +/- 3%; P < 0.001). Daily myofibrillar protein synthesis decreased by approximately 27% from 1.39 +/- 0.32%center dot d(-1) during HPA to 1.01 +/- 0.38%center dot d(-1) during SR (P < 0.05). Muscle atrophy F-box and myostatin mRNA expression were upregulated, whereas mechanistic target of rapamycin, p53, and PDK4 mRNA expression were downregulated after SR (P < 0.05). Conclusions One week of reduced physical activity and increased sedentary time substantially lowers daily myofibrillar protein synthesis rates in healthy young men.
机译：目的在寿命中，身体活动水平降低，时间花费久坐不动现。然而，关于这些行为变化对骨骼肌大规模调节的影响很少。本研究的主要目的是使用步骤减少模型来确定体育活动减少的影响，并增加健康青年男性日常肌原纤维蛋白综合率的久坐时间。方法11人（22 +/- 2 YR）完成了7天的习惯性身体活性（HPA），然后进行了7 d一步减少（SR）。使用氘水（（H2O）-H-2）方法与骨骼肌活检和每日唾液样品的收集结合，在HPA和Sr期间测定Myofibrillar蛋白质合成速率。通过实时逆转录定量聚合酶链反应（RT-QPCR）测定与肌肉质量调节相关和氧化代谢相关的所选蛋白质的基因表达。结果每日步骤计数在SR期间减少约91％（从每天的每天13,054 +/- 2763步或每天1192 +/- 330步; P <0.001），这导致了久坐久的日常活动的贡献增加了（ 73％+/- 6％至90％+/- 3％; p <0.001）。每日MyoFibrar蛋白合成在SR期间在HPA至1.01 +/- 0.38％中心点D（-1）期间从1.39 +/- 0.32％的中心点D（-1）降低约27％（-1）（P <0.05）。上调肌肉萎缩F盒和肌抑素mRNA表达，而雷帕霉素，P53和PDK4 mRNA表达的机械靶标在SR后下调（P <0.05）。结论一周的身体活动减少，久坐时间增加，健康年轻人的每日肌原纤维蛋白合成率显着降低。

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