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首页> 外文期刊>Medycyna Weterynaryjna >Molecular detection and seasonal distribution of lumpy skin disease virus in cattle breeds in Turkey
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Molecular detection and seasonal distribution of lumpy skin disease virus in cattle breeds in Turkey

机译：土耳其牧场品种皮肤病病毒的分子检测与季节分布

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The aim of this study was to investigate the prevalence and distribution patterns of LSDV infections in the provinces of northern Turkey, and to detect the factors influencing the epidemiology of LSD virus infections (age, breed, season, climate, geography, population dynamic, animal movement), as well as to assess the diagnostic value of the sampled materials in the diagnosis of LSDV infections. Lumpy skin disease (LSD) is an economically important cattle disease. The disease is endemic in many African countries, but outbreaks have also been reported in Turkey and the Middle East. In this study, a total of 564 samples (skin, blood and lung) from different cattle breeds (Jersey, Holstein-Friesian, Anatolian Black, Simmental and Brown Swiss) (n = 465) in the many herds suspected of lumpy skin disease virus (LSDV) infection as clinically and macroscopic pathologic remarks, housed in the 7 different provinces of northern Turkey, were used for gel based conventional polymerase chain reaction (PCR). LSDV nucleic acid was detected in 259 of 564 (45.92%) materials by PCR. According to the result of PCR, the LSDV infection was diagnosed in 54.62% (254/465) of the sampled animals. The diagnostic value of necropsy and clinical materials such as skin and lung were determined as more valuable diagnostic materials in the diagnosis of LSDV infection by PCR. Data showed that LSDV infection was widespread in the provinces of northern Turkey and that the prevalence of the infection in the region varies in accordance with factors such as geographical conditions (climate, season, location etc.) and the method of breeding.
机译：本研究的目的是探讨土耳其北部省份LSDV感染的患病率和分布模式，并检测影响LSD病毒感染流行病学的因素（年龄，品种，季节，气候，地理，人口动态，动物运动），以及评估采样材料在LSDV感染诊断中的诊断价值。 Lumpy皮肤病（LSD）是一种经济上重要的牛疾病。这种疾病在许多非洲国家流行，但也在土耳其和中东报告爆发。在这项研究中，共有564个样品（皮肤，血液和肺部）来自不同的牛品种（泽西，荷斯坦 - 弗里斯，anatolian黑色，Simmental和Brown Swiss）（n = 465）在许多牛群中涉嫌肿块皮肤病病毒（LSDV）感染作为临床和宏观病理言论，该言论在土耳其北部的7个不同省份，用于凝胶的常规聚合酶链反应（PCR）。通过PCR在564（45.92％）材料中检测到LSDV核酸。根据PCR的结果，LSDV感染被诊断为54.62％（254/465）的取样动物。尸检和临床材料如皮肤和肺等诊断值被确定为PCR诊断LSDV感染的更有价值的诊断材料。数据显示，LSDV感染在土耳其省份普遍存在，该地区感染的患病率根据地理条件（气候，季节，位置等）和繁殖方法等因素而变化。

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