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首页> 外文期刊>Microbial drug resistance: MDR : Mechanisms, epidemiology, and disease >Rhodomyrtus tomentosa Leaf Extract Inhibits Methicillin-Resistant Staphylococcus aureus Adhesion, Invasion, and Intracellular Survival in Human HaCaT Keratinocytes
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Rhodomyrtus tomentosa Leaf Extract Inhibits Methicillin-Resistant Staphylococcus aureus Adhesion, Invasion, and Intracellular Survival in Human HaCaT Keratinocytes

机译：rhodomyrtus tomentosa叶提取物抑制人类Hacat角蛋白细胞的耐甲氧菊抗性金黄色葡萄球菌粘附，侵袭和细胞内存活

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Methicillin-resistant Staphylococcus aureus (MRSA) has an ability to invade nonprofessional phagocytic cells, resulting in persistent infections and most likely host cell death. Series of our studies have claimed pronounced antibacterial efficacy of Rhodomyrtus tomentosa leaf extract. This study was to further investigate potency of the extract in intracellular killing of human HaCaT keratinocytes. Pretreatment of MRSA with the extract resulted in a remarkable reduction in the bacterial adhesion to HaCaT keratinocytes, compared with untreated control (p<0.001). In addition, at least 60% inhibition of the bacterial invasion into HaCaT cells was observed. Intracellular killing assay demonstrated that the extract exhibited strong antibacterial activity against intracellular MRSA at nontoxic concentrations (128mg/L), which may have resulted from the increase in bactericidal activity under phagolysosomal pH. Transmission electron microscopy displayed the effects of the extract on alterations in the bacterial cell morphology with cell lysis. Fluorescence microscopy revealed that the extract decreased MRSA-induced apoptosis in HaCaT cells. In addition, cytotoxicity of HaCaT cells caused by MRSA supernatant was reduced at least 50% by the extract. The potential activities of R. tomentosa extract may be useful in an alternative treatment of MRSA infections in slight acidic compartments, particularly skin infections.
机译：耐甲氧胞素抗金黄色葡萄球菌（MRSA）具有侵入非营养性吞噬细胞的能力，导致持续的感染和最有可能的宿主细胞死亡。我们的研究系列已声明Rhodomyrtus tomentosa叶提取物的明显抗菌效果。该研究是进一步研究提取物在人Hacat角蛋白细胞的细胞内杀死中的效力。与未治疗的对照相比，将MRSA的预处理与提取物具有显着的粘附性对HACAT角质形成细胞的显着降低（P <0.001）。此外，观察到至少60％的抑制细菌侵袭到HaCAT细胞中。细胞内杀伤测定证明，提取物在无毒浓度（128mg / L）下对细胞内MRSA的强抗菌活性表现出对细胞内MRSA的强烈抗菌活性，这可能是由于吞噬作用下的杀菌活性的增加。透射电子显微镜显示提取物对细胞裂解细菌细胞形态中的改变的影响。荧光显微镜表明，提取物降低了MRSA诱导的HACAT细胞凋亡。此外，由MRSA上清液引起的HaCAT细胞的细胞毒性通过提取物至少减少50％。 R. tomentosa提取物的潜在活动可用于替代治疗轻微酸性室中MRSA感染，特别是皮肤感染。

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