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首页> 外文期刊>Microbial drug resistance: MDR : Mechanisms, epidemiology, and disease >Whole-Genome Sequence Analysis of CTX-M Containing Escherichia coli Isolates from Retail Meats and Cattle in the United States
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Whole-Genome Sequence Analysis of CTX-M Containing Escherichia coli Isolates from Retail Meats and Cattle in the United States

机译：CTX-M含CTX-M的全基因组序列分析来自美国零售肉类和牛的大肠杆菌分离株

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In recent years, there have been increased reports on the detection of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Salmonella strains from food-producing animals and animal products in the United States. We characterized 18 ESBL E. coli isolates from cattle (n=5), chicken breast (n=5), ground turkey (n=6), ground beef (n=1), and pork chops (n=1) that were collected by the National Antimicrobial Resistance Monitoring System (NARMS) between 2011 and 2015. In vitro antimicrobial susceptibility testing was done against a panel of 14 antimicrobials followed by a secondary panel of 9 -lactam agents. Whole-genome sequencing was used to characterize the resistome, plasmids, and the genetic structures of the ESBL genes. All ESBL-producing E. coli isolates were resistant to at least three antimicrobial classes and carried various bla(CTX-M) genes. Most of the cattle and ground turkey isolates carried bla(CTX-M-27). In chicken breast isolates, bla(CTX-M-1) was present as part of an ISEcp1 transposition unit carried on a plasmid that shares sequence similarity with the backbone structure of the IncI plasmid. Isolates carrying the bla(CTX-M-14) and bla(CTX-M-15) genes, widely distributed in human clinical isolates, were also isolated. To our knowledge, this is the first report of the widely distributed bla(CTX-M-14) and bla(CTX-M-15) in E. coli isolates from retail meat samples in the United States. Different insertional sequences were identified upstream of these bla(CTX-Ms), including ISEcp1, IS26, and IS903-D. CTX-M in E. coli from food animals and retail chicken breast were often present on plasmids with other resistance genes. Other resistance genes identified included aadA, strA, strB, aac(3)-IId, aac(3)-VIa, aph(3)-Ic, bla(TEM), bla(HERA-3), floR, sul1, sul2, catA1, tetA, tetB, dfrA, and qacE. These data describe the emergence of CTX-M-carrying E. coli isolates in food animals and animal products monitored by NARMS program.
机译：近年来，有关于检测扩展β-内酰胺酶（ESBL）的报告 - 发放大肠杆菌和沙门氏菌和美国动物产品的动物产品和动物产品。从牛（n = 5），鸡胸肉（n = 5），地土耳其（n = 6），碎牛肉（n = 1），猪排（n = 1），鸡胸肉（n = 5），和猪排（n = 1）。 2011年和2015年间的国家抗菌抗性监测系统（NARMS）收集。在体外抗微生物易感性测试对14个抗微生物的面板进行，然后是9-酰胺剂的二级板。使用全基因组测序来表征ESBL基因的电阻，质粒和遗传结构。所有ESBL制造的大肠杆菌分离物抵抗至少三种抗微生物类别并携带各种BLA（CTX-M）基因。大多数牛和地面土耳其分离物携带BLA（CTX-M-27）。在鸡胸肉中，BLA（CTX-M-1）作为在载体的质粒上携带的ISECP1转置单元的一部分存在，其与INCI质粒的骨干结构共享序列相似性。还分离出携带BLA（CTX-M-14）和BLA（CTX-M-15）基因的BLA（CTX-M-15）基因的分离物也分离出分离。据我们所知，这是来自美国零售肉样本的大肠杆菌分离株广泛分布的BLA（CTX-M-14）和BLA（CTX-M-15）的第一个报告。在这些BLA（CTX-MS）的上游鉴定不同的插入序列，包括ISECP1，IS26和IS903-D。来自食物动物和零售鸡胸肉的大肠杆菌中的CTX-M通常存在于具有其他抗性基因的质粒上。鉴定的其他抗性基因包括Aada，Stra，STRB，AAC（3）-IID，AAC（3）-VIA，APH（3）-IC，BLA（TEM），BLA（Hera-3），Flor，Sul1，Sul2， cata1，teta，tetb，dfra和qace。这些数据描述了由NARMS计划监测的食物动物和动物产品中CTX-M携带的大肠杆菌分离物的出现。

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