Cellular localization, cloning and expression of Leishmania braziliensis Phospholipase A(1)

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Cellular localization, cloning and expression of Leishmania braziliensis Phospholipase A(1)

机译：Leishmania Braziliensis磷脂酶A（1）的细胞定位，克隆和表达

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Leishmaniasis is caused by several species of protozoan parasites of the genus Leishmania and represents an important global health problem. Leishmania braziliensis in particular is responsible of cutaneous and mucocutaneous forms of this parasitosis, with prevalence in Latin America. In the present work, we describe in L. braziliensis promastigotes and amastigotes the presence of a Phospholipase A(1) (PLA(1)) activity, an enzyme that catalyses extensive deacylation of phospholipids like phosphatidylcholine. In order to deepen the knowledge about L. braziliensis PLA(1), the cloning and expression of the gene that codifies for this enzyme was carried out in a baculovirus expression system with the obtaintion of a purified recombinant protein that displayed PLA(1) activity. Given that this is the first molecular and functional protein characterization of a PLA(1) in the Leishmania genus, we also performed a phylogenetic analysis of this gene throughout 12 species whose genome sequences were available. The results presented here will contribute to increase the knowledge about trypanosome phospholipases, which could be novel and valuable as potential targets to fight neglected diseases like Leishmaniasis.

机译：Leishmaniaisis是由Leishmania属的几种原生动物寄生虫引起的，并代表了一个重要的全球健康问题。 Leishmania Braziliensis特别是对这种寄生虫病的皮肤和粘膜肌肤形式负责，在拉丁美洲普遍存在。在目前的工作中，我们在L.Braziliensis Promastigotes和Amastigotes中描述了磷脂酶A（1）（PLA（1））活性的存在，一种酶催化磷脂酰胆碱等磷脂的广泛脱酰基化。为了加深关于L.Braziliensis PLA（1）的知识，在杆状病毒表达系统中进行了编纂该酶的基因的克隆和表达，溶于显示PLA（1）活性的纯化的重组蛋白。鉴于这是Leishmania属中PLA（1）的第一种分子和功能蛋白表征，我们还在整个基因组序列可获得的12种中进行该基因的系统发育分析。这里提出的结果将有助于提高磷蛋白酶体磷脂酶的知识，这可能是一种新颖的，有价值的潜在目标，潜在的靶向抗被忽视的疾病，如Leishmaniaisis。

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《Microbial Pathogenesis》 |2020年第2020期|共11页
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正文语种 eng
中图分类基础医学;
关键词
Leishmania braziliensis; Phospholipase A(1); Phosphatydilcholine; Baculovirus expression system; Phylogenetic analysis;

机译：Leishmania Braziliensis;磷脂酶A（1）;磷脂尿胆碱;杆状病毒表达系统;系统发育分析;

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4. Cellular localization, cloning and expression of Leishmania braziliensis Phospholipase A(1) [J] . Microbial Pathogenesis . 2020,第期

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Cellular localization, cloning and expression of Leishmania braziliensis Phospholipase A(1)

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