• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Microbial Pathogenesis >A novel Triticum durum Annexin 12 protein: Expression, purification and biological activities against Listeria monocytogenes growth in meat under refrigeration
【24h】

A novel Triticum durum Annexin 12 protein: Expression, purification and biological activities against Listeria monocytogenes growth in meat under refrigeration

机译：一种新的Triticum durum annexin 12蛋白：在制冷下对李斯特菌单核细胞增生的表达，纯化和生物学活性

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The present study was undertaken to investigate the expression, purification and biological activities of a novel Triticum durum Annexin 12 protein (TdAnn12). The findings indicated that the molecular weight of the purified TdAnn12 was estimated to 35 kDa. The purified TdAnn12 protein was modulated by, Methyl-jasmonate, and ethephon treatments. The purified TdAnn12 protein displayed good antimicrobial activities against 9 tested pathogenic bacteria. The antioxidant activities showed that TdAnn12 displayed an excellent DPPH scavenging ability with an IC50 of 8.33 mu g/ml and a strong Beta-carotene bleaching inhibition after 120 min of incubation with an IC50 of 2 mu g/ml the cytotoxic effects of the TdAnn 12 showed that HepG2 and MCF-7 were examined by MTT assay. The IC50 values were 250.35 and 400.25 mu g/ml for HepG2 and MCF-7 cells, respectively. The inhibitory effects of this TdAnn12 was assessed in vivo against Listeria monocytogenes, inoculated in minced beef meat at 6.10(5 )CFU/g amended with different concentrations of the purified TdAnn12 and stored at 4 degrees C for 21 days. Results showed an excellent inhibitory effect of TdAnn12 of this pathogenic bacterium at 4 degrees C. Overall, the TdAnn12 have potential application as active ingredients in food and pharmaceutical industry.
机译：本研究探讨了新型Triticum硬粒膜蛋白12蛋白（TDANN12）的表达，纯化和生物活性。结果表明，纯化的TDANN12的分子量估计为35kDa。纯化的TDANN12蛋白由甲基己酸酯和乙烯治疗调节。纯化的TDANN12蛋白对9个测试的致病细菌显示出良好的抗微生物活性。抗氧化活性表明，TDANN12展现出优异的DPPH清除能力，IC50为8.33μg/ ml，与20μm的IC50孵育120分钟后，培养20μmg/ mL TDANN 12的细胞毒性效应后的强β-胡萝卜素漂白抑制显示Hepg2和MCF-7通过MTT测定检查。 IC 50值分别为HepG2和MCF-7细胞250.35和400.25μg/ ml。该TDANN12的抑制作用在体内对李斯特菌单核细胞增生进行评估，在6.10（5）CFU / g的碎牛肉中接种，用不同浓度的纯化的TDANN12进行修复并在4℃下储存21天。结果表明，该致病菌的TDANN12在4摄氏度下表现出优异的抑制作用。总体而言，TDANN12具有作为食品和制药行业的活性成分的潜在应用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号