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首页> 外文期刊>Microbial Pathogenesis >Biocontrol activity and putative mechanism of Bacillus amyloliquefaciens (SF14 and SP10), Alcaligenes faecalis ACBC1, and Pantoea agglomerans ACBP1 against brown rot disease of fruit
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Biocontrol activity and putative mechanism of Bacillus amyloliquefaciens (SF14 and SP10), Alcaligenes faecalis ACBC1, and Pantoea agglomerans ACBP1 against brown rot disease of fruit

机译：Bacillus淀粉氨酰胺（SF14和SP10）的生物控制活性和推定机制，Alcaligenes Faecalis ACBC1和Pantoea Agglomerans ACBP1抗棕色腐烂疾病

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摘要

This study aimed at evaluating the antagonistic activity of 16 bacterial strains for the control of brown rot disease caused by Monilinia fructigena, and M. laxa under in vitro and a semi-commercial large-scale trial. These bacterial antagonists' belonging to the genera Alcaligenes, Bacillus, Brevibacteriwn, Pantoea, Pseudomonas, and Serratia were previously proven effective for control of fire blight of apple. The in vitro dual culture bioassay showed the highest inhibition rates of mycelial growth ranging from 55 to 95% and from 43 to 94% for M. fructigena and M. laxa, respectively. The in vivo bioassay showed moderate and strong inhibition for M. fructigena and M. laxa, respectively. The inhibition rates were dependent on incubation time as well as pathogen virulence. The free-cell bacterial filtrate revealed substantial mycelial growth inhibition ranging from 66 to 86%. The inhibition of conidial germination was from 32 to 78%, suggesting the involvement of metabolites in their biocontrol activity. The antifungal effect of the volatile compounds (VCOs) was observed for all bacteria with mycelial inhibition varying from 12 to 70%. Overall, their efficacy was substantially affected by the nature of the bacterial strains and the modes of action. Taken together, these results underscore that ACBC1 and SF14 for M. fructigena and SP10 and ACBP1 for M. laxa were the most effective bacterial strains. These strains were confirmed effective in a semi-commercial large-scale trial. Interestingly, their efficacies were found to be comparable to those of both commercial BCAs (B. subtilis Y1336 and P. agglomerans P10c), but slightly lower than thiophanate-methyl fungicide. The ability of most bacterial strains to produce lytic enzymes (Amylase, Protease or Cellulase) and lipopeptides (bacillomycin, fengycin, iturin and surfactin) was demonstrated by biochemical and molecular analyzes. Therefore, our findings suggest that the bacterial antagonists ACBC1, SF14, SP10 and ACBP1, have the potential to prevent brown rot disease.
机译：该研究旨在评估16种细菌菌株的拮抗活性，用于控制Monilinia Fructia植物的棕色腐疾病，以及在体外和半商业大规模试验下的M. Laxa。这些细菌拮抗剂的属于属alcaligenes，芽孢杆菌，Brevibacteriwn，Pantoea，假单胞菌和血清症是有效的，对苹果的火焰枯萎有效。体外二元培养生物测定菌培养率的最高抑制率，菌丝体增长的抑制率分别为55％至95％，分别为M.Muctena和M. Laxa的43％至94％。体内生物测定分别显示出对M. Fructiena和M. Laxa的中等和强烈的抑制。抑制率取决于孵育时间以及病原体毒力。自由细胞细菌滤液显示出大量的菌丝体生长抑制范围为66至86％。分析萌发的抑制为32％至78％，表明代谢物在其生物控制活性中的参与。对所有细菌的菌丝抑制的所有细菌都观察到挥发性化合物（VCO）的抗真菌效应，从12-70％变化。总体而言，它们的疗效基本上受细菌菌株的性质和作用方式的影响。总之，这些结果强调了用于M.果酱和SP10的ACBC1和SF14和M.LAXA的ACBP1是最有效的细菌菌株。这些菌株在半商业大规模试验中确认有效。有趣的是，发现它们的疗效与商业BCAS（B.枯草芽孢杆菌Y1336和P.聚集体P10C）相当，但略低于噻吩 - 甲基杀真菌剂。通过生化和分子分析证明了大多数细菌菌株产生裂解酶（淀粉酶，蛋白酶，纤维素酶）和脂肽（甲嘧霉素，枫霉素，紫蛋白和SURFACTIN）的能力。因此，我们的研究结果表明，细菌拮抗剂ACBC1，SF14，SP10和ACBP1具有预防褐变腐败的可能性。

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