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首页> 外文期刊>Microbial Pathogenesis >Evidences of differential endoproteolytic processing on the surfaces of Mycoplasma hyopneumoniae and Mycoplasma flocculare
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Evidences of differential endoproteolytic processing on the surfaces of Mycoplasma hyopneumoniae and Mycoplasma flocculare

机译：差动内含药物加工在支原藓疫苗和支原体絮凝物的表面上的证据

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Mycoplasma hyopneumoniae and Mycoplasma flocculare are genetic similar bacteria that colonize the swine respiratory tract. However, while M. hyopneumoniae is a pathogen that causes porcine enzootic pneumonia, M. flocculare is a commensal. Adhesion to the respiratory epithelium is mediated by surface-displayed adhesins, and at least some M. hyopneumoniae adhesins are post-translational proteolytically processed, producing differential proteoforms with differential adhesion properties. Based on LC-MS/MS data, we assessed differential proteolytic processing among orthologs of the five most abundant adhesins (p97 and p216) or adhesion-related surface proteins (DnaK, p46, and ABC transporter xylose-binding lipoprotein) from M. hyopneumoniae strains 7448 (pathogenic) and J (non-pathogenic), and M. flocculare. Both surface and cytoplasmic non-cryptic cleavage events were mapped and compared, and antigenicity predictions were performed for the resulting proteoforms. It was demonstrated that not only bona fide adhesins, but also adhesion-related proteins undergo proteolytical processing. Moreover, most of the detected cleavage events were differential among M. hyopneumoniae strains and M. flocculare, and also between cell surface and cytoplasm. Overall, our data provided evidences of a complex scenario of multiple antigenic proteoforms of adhesion-related proteins, that is differential among M. hyopneumoniae strains and M. flocculare, altering the surface architecture and likely contributing to virulence and pathogenicity.
机译：支原体吞咽母细胞和支原体Flocculare是遗传相似的细菌，可殖民呼吸道。然而，虽然M.肺炎素是导致猪Enzootic肺炎的病原体，但M. Flocculare是一个共生。对呼吸上皮的粘附性由表面展示的粘附素介导，并且至少一些M.吞噬蛋白是翻译后蛋白水解处理的，产生具有差异粘附性的微分蛋白质常规。基于LC-MS / MS数据，我们评估了来自M. yopneumoniae的五种最丰富的粘附素（P97和P216）或粘附相关表面蛋白（DNAK，P46和ABC转运蛋白结合脂蛋白结合脂蛋白）的差异蛋白水溶液菌株7448（致病）和J（非致病性）和M. flocculare。映射并比较了表面和细胞质非脊布裂解事件，对所得蛋白质常规进行抗原性预测。据证明，不仅是真绒粘连蛋白，而且还有粘附相关的蛋白质进行蛋白水解加工。此外，大部分检测到的切割事件是M.吞噬菌株和M. flocculare之间的差异，以及细胞表面和细胞质之间。总体而言，我们的数据提供了粘附相关蛋白的多种抗原蛋白质的复杂场景的证据，即M.吞咽菌株和M. flocculare之间的差异，改变表面架构并可能有助于毒力和致病性。

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