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首页> 外文期刊>Microbial Pathogenesis >Transcriptome sequencing analysis of porcine MDM response to FSL-1 stimulation
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Transcriptome sequencing analysis of porcine MDM response to FSL-1 stimulation

机译：猪MDM对FSL-1刺激的转录物测序分析

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Mycoplasma infection can cause many diseases in pigs, resulting in great economic losses in pork production. Innate immune responses are thought to play critical roles in the pathogenesis of mycoplasma disease. However, the molecular events involved in immune responses remain to be determined. Hence, the object of this study was to use RNA-Seq to investigate the gene expression profiles of the innate immune response mediated by FSL-1 in pig monocyte-derived macrophages (MDMs). The results revealed that 1442 genes were differentially expressed in the FSL-1 group compared with the control groups, of which 777 genes were upregulated and 665 genes were downregulated. KEGG pathway analysis showed that the upregulated genes were mainly involved in innate immune-related pathways including the TNF signaling pathway, cytokine-cytokine receptor interaction, Toll-like receptor signaling pathway, Jak-STAT signaling pathway, chemokine signaling pathway, NOD-like receptor signaling pathway and NF-kappa B signaling pathway. The downregulated genes were only involved in the cGMP-PKG signaling pathway and glycerophospholipid metabolism. Our results showed that FSL-1 stimulation activated the TLR2 signaling pathway and resulted in diverse inflammatory responses. FSL-1 induced the transcription of numerous protein-coding genes involved in a complex network of innate immune-related pathways. We speculate that TNF, IL1B, IL6, NFKB1, NFKBIA, CXCL2, CXCL8, CXCL10, CCL2, CCL4 and CCL5 were the most likely hub genes that play important roles in the above pathways. This study identified the differentially expressed genes and their related signaling pathways, contributing to the comprehensive understanding of the mechanisms underlying host-pathogen interactions during mycoplasma infection and providing a reference model for further studies.
机译：支原体感染可能导致猪的许多疾病，导致猪肉生产中的经济损失很大。先天免疫应答被认为在支原体病的发病机制中发挥着关键作用。然而，仍有待确定免疫应答所涉及的分子事件。因此，本研究的目的是使用RNA-SEQ研究在猪单核细胞衍生的巨噬细胞（MDMS）中由FSL-1介导的先天免疫应答的基因表达谱。结果表明，与对照组相比，在FSL-1组中，1442个基因在FSL-1组中表达，其中上调777个基因，下调665个基因。 Kegg途径分析表明，上调基因主要涉及包括TNF信号通路，细胞因子 - 细胞因子受体相互作用，Toll样受体信号通路，JAK-STAT信号通路，趋化因子信号通路，NOD样的受体的先生免疫相关途径。信号通路和NF-Kappa发信号通路。下调基因仅参与CGMP-PKG信号通路和甘油磷脂代谢。我们的结果表明，FSL-1刺激激活了TLR2信号通路，导致各种炎症反应。 FSL-1诱导了涉及涉及先天性免疫相关途径的复杂网络的许多蛋白质编码基因的转录。我们推测TNF，IL1B，IL6，NFKB1，NFKBIA，CXCL2，CCL8，CXCL10，CCL2，CCL4和CCL5是最可能在上述途径中发挥重要作用的最可能的枢纽基因。该研究确定了差异表达的基因及其相关的信号通路，有助于综合了解支原体感染期间宿主病原体相互作用的机制，并为进一步研究提供参考模型。

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