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首页> 外文期刊>Microbial Pathogenesis >Characterization and phytostimulatory activity of bacteria isolated from tomato (Lycopersicon esculentum Mill.) rhizosphere
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Characterization and phytostimulatory activity of bacteria isolated from tomato (Lycopersicon esculentum Mill.) rhizosphere

机译：番茄（Lycopersicon Esculentum Mill）分离的细菌的特征和植物刺激活性。根际

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Replacing agrochemicals with plant growth promoting bacteria (PGPB) may offset some of the environmental impacts of food production. The objectives of this study were to (1) isolate and characterize bacterial strains from tomato rhizosphere, including root, shoot and leaf, (2) select and identify the most promising PGPB strains, (3) verify the phytostimulatory activity and mineral uptake potential of selected strains. Bacterial strains isolated from tomato rhizosphere, were screened for phosphorous (P) solubilization, production of indole acetic acid (IAA), amylase activity, antibiotic resistance, and quick test strip (QTS) for biochemical characterization. The tested strains, positive for all five of these assays were selected for molecular identification and subjected to greenhouse growth trails with tomato and mung bean. Two strains were selected and identified as Bacillus cereus (B. cereus) isolated from rhizosphere and Klebsiella variicola (K. variicola) isolated from root endosphere using 16s rRNA sequences. Both strains produced IAA, gibberellic acid (GA3) and kinetin, however B. cereus showed potential GA3 and IAA production as compared to K. variicola. In tomato, only one growth variable (shoot length) was increased over the control by one of the selected bacterial strains (B. cereus). In mung bean, inoculation with either strain B. cereus or K. variicola increased shoot length and dry weight. Moreover, our results showed that the use of PGPB significantly increased plant growth and Fe, Zn, Ca, Mg, Cu, Na and K contents of plants. It seems that evaluated strains had a higher ability in boosting plant growth and higher yield.
机译：用植物生长促进细菌（PGPB）取代农用化学品可能抵消食品生产的一些环境影响。本研究的目的是（1）分离和表征来自番茄根际的细菌菌株，包括根，芽和叶，（2）选择并鉴定最有前途的PGPB菌株，（3）验证植物刺激性活性和矿物吸收潜力选定的菌株。从番茄根际分离的细菌菌株进行磷（P）溶解，生产吲哚乙酸（IAA），淀粉酶活性，抗生素抗性和快速试验条（QTS）的生化表征。选择测试的菌株，对所有五种测定的阳性进行分子鉴定并进行温室生长迹线与番茄和绿豆。选择两种菌株并鉴定为从根际和Klebsiella Variicola（K.Variicola）中分离的芽孢杆菌（B.Cereus）使用16S rRNA序列与根腹圈中分离的芽孢杆菌（K.Variicola）。两种菌株产生IAA，凝胶酸（GA3）和KINETIN，然而B.培养物与K.Variicola相比，培养物显示出潜在的GA3和IAA产生。在番茄中，通过一个所选细菌菌株（B.Cereus）的对照，仅增加一个生长变量（射击长度）。在绿豆中，用菌株B.癌症或K. Variicola接种，增加枝条长度和干重。此外，我们的研究结果表明，PGPB的使用显着增加了植物生长和Fe，Zn，Ca，Mg，Cu，Na和K含量的植物。似乎评估的菌株促进了植物生长和更高产量的能力较高。

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