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首页> 外文期刊>Microbial Pathogenesis >Assembly of pigeon circovirus-like particles using baculovirus expression system
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Assembly of pigeon circovirus-like particles using baculovirus expression system

机译：使用Baculovirus表达系统组装鸽子圆锥像颗粒

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Pigeon circovirus (PiCV) is able to infect racing and meat pigeons of all ages and is a key factor that triggers young pigeon disease syndrome (YPDS). PiCV vaccine research has been impeded because PiCV cannot be grown or propagated in cell cultures. Virus-like particles (VLPs), which can be generated by a wide range of expression systems, have been shown to have outstanding immunogenicity and constitute promising vaccines against a wide range of pathogens. Cap protein, which contains neutralizing antibody epitopes, is the only capsid protein of PiCV. In this study, the baculovirus expression system was utilized to express the PiCV Cap protein, which was self-assembled into VLPs with a spherical morphology and diameters of 15-18 nm. Specific antibodies against the Cap protein were induced after BALB/c mice immunized intramuscularly (i.m.) with VLPs combined with adjuvant. Based on these findings, PiCV VLPs may be a promising candidate vaccine against PiCV.
机译：Pigeon Circovirus（PICV）能够感染所有年龄段的赛车和肉鸽，是触发幼鸽症综合征（YPDS）的关键因素。 PICV疫苗研究已经受阻，因为PICV不能在细胞培养物中生长或繁殖。 已经显示出可通过各种表达系统产生的病毒样颗粒（VLP）具有突出的免疫原性，并且构成了对各种病原体的有前途的疫苗。 含有中和抗体表位的帽蛋白是PICV的唯一衣壳蛋白。 在该研究中，利用杆状病毒表达系统表达PICV盖蛋白，其具有15-18nm的球形形态和直径的自组装成VLP。 在肌肉内（即）与佐剂结合佐剂后，在肌肉内（I.M.）免疫的BALB / C小鼠之后诱导对帽蛋白的特异性抗体。 基于这些发现，PICV VLP可能是对PICV的有希望的候选疫苗。

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