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首页> 外文期刊>Microbial Pathogenesis >Understanding the pathogenesis of Flavobacterium psychrophilum using the rainbow trout monocyte/macrophage-like cell line, RTS11, as an infection model
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Understanding the pathogenesis of Flavobacterium psychrophilum using the rainbow trout monocyte/macrophage-like cell line, RTS11, as an infection model

机译：用虹鳟鱼单核细胞/巨噬细胞样细胞系RTS11了解黄芪心肌粒细胞的发病机制，作为感染模型

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The life cycle of Flavobacterium psychrophilum (Fp), the causative agent of bacterial coldwater disease (BCWD) and rainbow trout fry syndrome (RTFS), appears to involve interactions with spleen and head kidney macrophages. To develop an in vitro model for studying this, F. psychrophilum was incubated with a rainbow trout splenic monocyte/macrophage-like cell line (RTS11) and fundamental macrophage functions evaluated. The animal cell basal medium, L15, supplemented with bovine serum (FBS) supports RTS11 maintenance, and surprisingly, L15 with 2% FBS (L15/FBS) also supported F. psychrophilum growth. L15/FBS in which the bacteria had been grown is referred to as F. psychrophilum conditioned medium (FpCM). Adding FpCM to RTS11 cultures caused a small, yet significant, percentage of cells to die, many cells to become more diffuse, and phagocytosis to be temporarily reduced. FpCM also significantly stimulated transcript expression for pro-inflammatory cytokines (IL-1 beta, TNF alpha and IL-6) and the anti-inflammatory cytokine (IL-10) after one day of exposure but this upregulation rapidly declined over time. Adding live F. psychrophilum to RTS11 cultures also altered the cellular morphology and stimulated cytokine expression more profoundly than FpCM. Additionally, the phagocytic activity of RTS11 was also significantly impaired by live F. psychrophilum, but not to the same extent as when exposed to FpCM. Adding heat-killed bacteria to RTS11 cultures elicited few changes. These bacteria/RTS11 co-cultures should be useful for gaining a deeper understanding of the pathogenesis of F. psychrophilum and may aid in the development of effective measures to prevent infection and spread of this troublesome disease.
机译：意大利气味（FP）的生命周期（FP），细菌冷水疾病（BCWD）和彩虹鳟鱼霉菌综合征（RTFS）的致病剂似乎涉及与脾脏和头肾巨噬细胞的相互作用。为了开发学习的体外模型，F.Scashcrophilum与虹鳟鱼单核细胞/巨噬细胞样细胞系（RTS11）孵育和评估的基本巨噬细胞函数。辅助牛血清（FBS）的动物细胞基体培养基L15支持RTS11维持，令人惊讶的是，L15具有2％FBS（L15 / FBS）也支持F.Cechentrophilum生长。 L15 / FBS，其中生长的细菌被称为F.Ciscrophilum条件培养基（FPCM）。将FPCM添加到RTS11培养物引起的小，但显着，百分比的细胞死亡，许多细胞变得更加弥漫，并且吞噬吞噬作用暂时降低。 FPCM还显着刺激了促炎细胞因子（IL-1β，TNFα和IL-6）的转录表达和在暴露一天后的抗炎细胞因子（IL-10），但这种上调随着时间的推移迅速下降。添加活性F.Cyscophilum至RTS11培养也改变了细胞形态并刺激了细胞因子表达比FPCM更深刻。此外，RTS11的吞噬活性也受到活性F.Scasscrophilum的显着损害，但在暴露于FPCM时没有与相同的程度。将热杀死的细菌添加到RTS11培养物中引发了几种变化。这些细菌/ RTS11共培养物应该有助于获得对F.心理粒细胞发病机制的更深入了解，并有助于开发有效措施，以防止这种麻烦疾病的感染和传播。

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