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首页> 外文期刊>Microbial Pathogenesis >Antimicrobial mechanism of luteolin against Staphylococcus aureus and Listeria monocytogenes and its antibiofilm properties
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Antimicrobial mechanism of luteolin against Staphylococcus aureus and Listeria monocytogenes and its antibiofilm properties

机译：黄氏菌素对金黄色葡萄球菌的抗菌机制及李斯特菌单核细胞增生及其抗生素性能

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摘要

Luteolin (LUT) is a naturally occurring compound found in a various of plants. Few recent studies have reported LUT antimicrobial activities against bacterial pathogens, however, the fundamental LUT mediated antimicrobial mechanism has never been elucidated. This study aimed to investigate the antimicrobial activities of LUT and its mode of action against Staphylococcus aureus and Listeria monocytogenes, either as planktonic cells or as biofilms. Here, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) of LUT against S. aureus and L. monocytogenes were determined using the broth microdilution method, and the antimicrobial mode of LUT was elucidated by evaluating the variations in both cell membrane integrity and cell morphology. Moreover, the biofilm inhibition was measured by crystal violet staining assay, while its qualitative imaging was achieved by confocal laser scanning microscope and field emission scanning electron microscope. MIC and MBC values of LUT against S. aureus were 16-32 and 32-64 mu g/mL, and 32-64 and 64-128 mu g/mL for L. mono-cytogenes. LUT destroyed the cell membrane integrity, as evidenced by a significant increase in the number of non-viable cells, and well-defined variations in cell morphology. Moreover, LUT presented robust inhibitory effects on the biofilm formation, enhanced antibiotics diffusion within biofilms and killed efficiently mono- and dual-species biofilm cells. Overall, LUT demonstrates potent antimicrobial properties on planktonic and biofilm cells, and the biofilm formation, and thus has the potential use as a natural food preservative in foods.
机译：叶黄素（LUT）是在各种植物中发现的天然存在的化合物。最近的几项研究报告了针对细菌病原体的LUT抗菌活性，然而，根本的LUT介导的抗微生物机制从未被阐明过。本研究旨在探讨LUT及其对金黄色葡萄球菌和李斯特菌单核细胞生成的抗菌活性的抗菌活性，作为氏菌细胞或作为生物膜。这里，使用肉汤微量稀释方法测定Lut抗黄绿骨和L.单核细胞增生的最小抑制浓度（MIC）和最小杀菌浓度（MBC），通过评价细胞膜中的变化来阐明LUT的抗微生物模式完整性和细胞形态。此外，通过晶体紫染色测定法测量生物膜抑制，而通过共聚焦激光扫描显微镜和现场发射扫描电子显微镜实现其定性成像。 Lut的MIC和MBC值对抗金黄色葡萄球菌为16-32和32-64μg/ ml，32-64和64-128μg，L.单细胞生成。 LUT破坏了细胞膜完整性，如不可行细胞数量的显着增加，以及细胞形态的明确变异。此外，LUT呈现对生物膜形成的鲁棒抑制作用，增强生物膜内的抗生素扩散，并有效地杀死了单一和双种生物膜细胞。总体而言，LUT在浮游和生物膜细胞和生物膜形成上证明了有效的抗菌性质，因此具有作为食物中的天然食品防腐剂的潜在用途。

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