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首页> 外文期刊>Microbial Pathogenesis >Neuropathology mediated through caspase dependent extrinsic pathway in goat kids naturally infected with PPRV
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Neuropathology mediated through caspase dependent extrinsic pathway in goat kids naturally infected with PPRV

机译：神经病理学通过Caspase依赖外在途径介导的山羊儿童自然感染PPRV

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Peste des petits ruminant (PPR), a highly contagious viral disease of small ruminants, is characterized by erosive stomatitis and pneumo-enteritis. However, its neurovirulence potential as observed with other morbilliviruses has not been fully investigated. The present study describes the neuropathological alterations induced by PPR virus through apoptotic pathway. A total number of 12 carcasses of local breed goat kids of either sex were received for postmortem examination. The clinical history was described as symptoms of mucopurulent nasal discharge, high to low grade fever, erosive stomatitis, dyspnoea and profuse watery diarrhoea followed by mortality of 35 goat kids within a week. The pathoanatomical lesions and immunohistochemical demonstration of PPRV antigen in lungs, intestine, spleen and lymph nodes confirmed PPR disease in goats. Grossly, five brain specimens showed moderate to severe leptomeningeal congestion during necropsy. Microscopically, brain sections showed leptomeningitis and nonsuppurative encephalitis characterized by vascular congestion, haemorrhages in the parenchyma, perivascular cuffing with mild to moderate mononuclear cells (mainly lymphocytes and few macrophages), focal to diffuse microgliosis, neuronal degeneration, satellitosis and neuronophagia. Immunolabelling of viral antigen was observed in the cytoplasm of neurons and glial cells. The RT-PCR amplification of N gene fragment also confirmed the presence of PPRV in the brain. The strong immunoreactivity of Caspase-3, Caspase-8 and comparatively lower expression of caspase-9 along with the absence of any reactivity for Apaf-1 antigen in the brain sections indicated the role of caspase dependent extrinsic pathway in inducing neuropathological changes. The presence of apoptotic neurons in the brain by TUNEL assay further confirmed the apoptosis and strong immunoreactivity of iNOS in neurons which suggested the generation of oxidative stress, that might have induced the apoptosis. The overall findings confirm the neurovirulence potential of PPR virus, via the extrinsic pathway of apoptosis, in natural cases of PPR disease in goat kids.
机译：Peste des Petits反刍动物（PPR）是一种高度传染性的小反刍动物病毒性疾病，其特征在于糜烂性口炎和肺炎。然而，与其他Morbillivuses观察到的其神经血管血管潜力尚未得到充分调查。本研究描述了PPR病毒通过凋亡途径诱导的神经病理改变。在淘汰后，收到了两种性别山羊儿童的12个胴体总数。临床病史被描述为粘性鼻涕的症状，高于低级发热，腐蚀性口炎，呼吸困难和大量水汪汪的腹泻，然后在一周内将35个山羊儿童的死亡率。肺癌，肠，脾胃和淋巴结中PPRV抗原的病理疾病和免疫组织化学证明证实了山羊PPR病。总，五个脑标本显示尸检期间中度至严重的百分之肌肌肉充血。显微镜，脑切片表现出含有血管性充血的脑膜炎和非植物脑炎，疾病中的出血，羽毛肿胀与轻度至中度单核细胞（主要是淋巴细胞和少量巨噬细胞），局灶于弥漫性微细胞源，神经元变性，卫星病症和神经元。在神经元和神经胶质细胞的细胞质中观察到病毒抗原的免疫标签。 N基因片段的RT-PCR扩增也证实了脑中PPRV的存在。 Caspase-3，Caspase-8和Caspase-9的相对较低的Caspase-9的强免疫反应性以及脑切片中Apaf-1抗原的任何反应性的情况表明Caspase依赖性外在途径在诱导神经病理学变化中的作用。 TUNEL测定的脑中凋亡神经元的存在进一步证实了神经元中INOS的细胞凋亡和强烈免疫反应性，提出了可能诱导细胞凋亡的氧化应激。总体研究结果证实了PPR病毒的神经血管尿，通过凋亡的外在途径，在山羊儿童的PPR病的自然案例中。

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