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首页> 外文期刊>Microbial Pathogenesis >Transcription factor VmSeb1 is required for the growth, development, and virulence in Valsa mali
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Transcription factor VmSeb1 is required for the growth, development, and virulence in Valsa mali

机译：Valsa Mali中的生长，发育和毒力需要转录因子VmseB1

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摘要

Transcription factor Seb1 contains two C2H2 zinc finger motifs which are similar to the Msn2/4 of Saccharomyces cerevisiae. The homologous proteins of Seb1 function to regulate the response to various stresses or decomposing and utilizing pectin in some fungi. In this study, we characterized a homologue of Seb1 gene, VmSeb1, in Valsa mali, which causes a highly destructive bark disease on apple. VmSeb1 deletion mutant showed a drastic reduction in growth rate in vitro. It is also important for conidiation because VmSeb1 deletion mutant formed more pycnidia on PDA medium. Deletion mutant of VmSeb1 increased melanin genes expression. In addition, the sensitivity to oxidative stress increased and cell wall inhibitor in VmSeb1 deletion mutant, as its growth was more severely inhibited by H2O2 and Congo red than that in the wild-type. The virulence assay showed that the lesion length caused by the VmSeb1 deletion mutant was smaller compared to wild-type on detached apple twigs. However, expression of pectinase genes and pectinase activity in deletion mutant were the same as those of the wild-type during infection. These results indicate that VmSeb1 plays important roles in growth, asexual development, response to oxidative stress, maintenance of cell wall integrity, and virulence. However, VmSeb1 is not involved in the regulation of pectinase genes expression in V. mali.
机译：转录因子SeB1含有两个C 2 H 2锌指基象，其类似于酿酒酵母的MSN2 / 4。 SEB1的同源蛋白质可以调节对各种应力或分解并利用果胶在一些真菌中的反应。在这项研究中，我们在ValsaMali中表征了SEB1基因，VMSEB1的同源物，它会在苹果上引起高度破坏性的树皮疾病。 VMSEB1缺失突变体显示出体外生长速率的急剧降低。共复合也很重要，因为VMSEB1缺失突变体在PDA培养基上形成了更多的Pycnidia。 VmseB1的缺失突变体增加了黑色素基因的表达。此外，在VMSEB1缺失突变体中对氧化应激增加和细胞壁抑制剂的敏感性，因为其生长受到H 2 O 2和刚果红色比野生型更严重的抑制。毒力测定表明，与野生型在拆开的苹果树枝上相比，由VmseB1缺失突变体引起的病变长度较小。然而，脱毛突变体中果胶酶基因和果胶酶活性的表达与感染期间野生型的表达相同。这些结果表明，VMSEB1在生长，无性发展，对氧化应激的反应，维持细胞壁完整性和毒力方面起着重要作用。然而，VMSEB1没有参与在V.Mali中的果胶酶基因的调节。

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  • 来源
    《Microbial Pathogenesis》 |2018年第2018期|共7页
  • 作者

    Wu Yuxing; Xu Liangsheng; Yin Zhiyuan; Feng Hao; Huang Lili;

  • 作者单位

    Northwest A&

    F Univ Coll Plant Protect China Australia Joint Res Ctr Abiot &

    Biot Stress State Key Lab Crop Stress Biol Arid Areas Yangling 712100 Shaanxi Peoples R China;

    Northwest A&

    F Univ Coll Plant Protect China Australia Joint Res Ctr Abiot &

    Biot Stress State Key Lab Crop Stress Biol Arid Areas Yangling 712100 Shaanxi Peoples R China;

    Northwest A&

    F Univ Coll Plant Protect China Australia Joint Res Ctr Abiot &

    Biot Stress State Key Lab Crop Stress Biol Arid Areas Yangling 712100 Shaanxi Peoples R China;

    Northwest A&

    F Univ Coll Plant Protect China Australia Joint Res Ctr Abiot &

    Biot Stress State Key Lab Crop Stress Biol Arid Areas Yangling 712100 Shaanxi Peoples R China;

    Northwest A&

    F Univ Coll Plant Protect China Australia Joint Res Ctr Abiot &

    Biot Stress State Key Lab Crop Stress Biol Arid Areas Yangling 712100 Shaanxi Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 基础医学;
  • 关键词

    Malus domestica; Deletion mutant; Conidiation; Oxidative stress; Pectinase;

    机译：Malus domestica;删除突变体;结合;氧化应激;果胶酶;

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