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Nutritional interference for phenotypic biofilm quantification in Aeromonas spp. isolates containing the fla gene

机译：Aeromonas SPP中表型生物膜定量的营养干扰。 分离株含有FLA基因

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The Aeromonas genus has several virulence factors associated with the development of diseases in aquatic organisms, leading to losses in aquaculture. One of these factors is the flagella's formation which allows the biofilm's formation that provides the microorganisms a greater pathogenicity, greater protection to certain substances such as antibiotics. The aim of the study was to verify the presence of the fla gene, related to biofilm production in isolates of Aeromonas spp. from fishes and also to determine the best quantification condition of phenotypic biofilm production in vitro. Polymerase Chain Reactions were performed to obtain the amplification of the region comprising the fla gene. To determine the best condition for the production biofilm, the microplate adhesion test was carried out under different concentrations of TSB broth and it combined with glucose. Of the 43 isolates of Aeromonas spp. analyzed, 28 were positive for the fia gene and, in the quantification of the biofilm, all these were able to form biofilm in the TSB broth without dilution and without addition of glucose, being this the best condition tested. It was observed that the isolates of Aeromonas spp. analyzed have potential for biofilm formation, and hence potential for virulence.
机译：Aeromonas属的属性有几种与水生生物疾病的发展相关的毒力因子，导致水产养殖损失。这些因素之一是鞭毛的形成，这使得生物膜的形成提供了更大的致病性，对某些物质如抗生素的形成更大的保护。该研究的目的是验证FLA基因的存在，与Aeromonas SPP的分离株中的生物膜产生有关。来自鱼类，并确定体外表型生物膜生产的最佳定量条件。进行聚合酶链反应以获得包含FLA基因的区域的扩增。为了确定生产生物膜的最佳条件，在不同浓度的TSB肉汤下进行微孔板粘附试验，与葡萄糖相结合。在Aeromonas SPP的43个分离物中。分析，28个为FIA基因阳性，在毕杂膜的定量中，所有这些都能够在TSB肉汤中形成生物膜，而无需稀释并且不添加葡萄糖，是测试的最佳状态。观察到Aeromonas SPP的分离物。分析具有生物膜形成的可能性，因此毒力的潜力。

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