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Great effect of porin(aha) in bacterial adhesion and virulence regulation in Aeromonas veronii

机译：Porin（AHA）在AeroMonas Veronii中的细菌粘附和毒力调节中的大效果

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摘要

Aeromonas veronii is a serious pathogen which can infect mammals and aquatic organisms and causes irreparable damage to fish aquaculture. It has been demonstrated that adhesion to host surface and cells is the initial step in bacterial pathogenesis. Previous study found that bacterial weaken motility probably caused by the absence of flagellar related genes. In this study, we generated the aha deletion and complementary strains and found that two strains can be stably inherited for more than 50 generations. No significant change was found in the growth of mutant Delta aha. But the ability of biofilm formation, the adhesion and invasion to EPC cells significantly decreased for 3.7-fold and 2.3-fold respectively. Due to aha gene deletion, the stability of A. veronii flagellar was severely declined and the mutant Delta aha with no mobility. Compared with the wild-type TH0426, the pathogenicity of A. veronii aha-deleted strain to zebrafish and mice reduced significantly and virulence attenuated severely. Cytotoxicity experiment also proved that mutant Delta aha showed much weaker virulence at the same time infection. The consequences declared that the stability of flagellar decreased severely with porin missing and lost the motility. Porin regulated by aha gene is essential for the adhesion and virulence of A. veronii. Thence, the mutant Delta aha of A. veronii provides an important tool for further concentration on the pathogenic mechanism of A. veronii.
机译：Aeromonas Veronii是一种严重的病原体，可以感染哺乳动物和水生生物，并对鱼类水产养殖造成无法弥补的损害。已经证明对宿主表面和细胞的粘附是细菌性发病机制的初始步骤。以前的研究发现，细菌减弱的动力可能是由于没有鞭毛相关基因而引起的。在这项研究中，我们产生了AHA缺失和互补菌株，发现两个菌株可以稳定地遗传超过50代。在突变体Delta Aha的生长中没有发现显着变化。但是生物膜形成，粘附性和侵袭对EPC细胞的能力分别显着降低3.7倍和2.3倍。由于AHA基因缺失，A.Veronii鞭毛的稳定性严重下降，并且没有移动性的突变δAha。与野生型Th0426相比，A.Veronii Aha缺失的斑马鱼和小鼠的致病性显着减少，严重减少了毒力。细胞毒性实验还证明了突变δαHA在同一时间感染表现出微弱的毒力。后果宣布，鞭打的稳定性严重减少了豪猪，失去了动力。 AHA基因调节的茯苓对于A.Veronii的粘附和毒力是必不可少的。因此，A.Veronii的突变体Delta Aha提供了一种重要的工具，用于进一步浓缩A.Veronii的致病机制。

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  • 来源
    《Microbial Pathogenesis》 |2019年第2019期|共10页
  • 作者

    Song Hai-Chao; Kang Yuan-Huan; Zhang Dong-Xing; Chen Long; Qian Ai-Dong; Shan Xiao-Feng; Li Ying;

  • 作者单位

    Jilin Agr Univ Coll Anim Sci &

    Technol 2888 Xincheng St Changchun 130118 Jilin Peoples R China;

    Jilin Agr Univ Coll Anim Sci &

    Technol 2888 Xincheng St Changchun 130118 Jilin Peoples R China;

    Jilin Agr Univ Coll Anim Sci &

    Technol 2888 Xincheng St Changchun 130118 Jilin Peoples R China;

    Jilin Agr Univ Coll Anim Sci &

    Technol 2888 Xincheng St Changchun 130118 Jilin Peoples R China;

    Jilin Agr Univ Coll Anim Sci &

    Technol 2888 Xincheng St Changchun 130118 Jilin Peoples R China;

    Jilin Agr Univ Coll Anim Sci &

    Technol 2888 Xincheng St Changchun 130118 Jilin Peoples R China;

    Jilin Agr Univ Coll Anim Sci &

    Technol 2888 Xincheng St Changchun 130118 Jilin Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 基础医学;
  • 关键词

    Flagellar; Motility; Aeromonas veronii; Biofilm; Virulence;

    机译：鞭毛;摩托车;Aeromonas Veronii;生物膜;毒力;

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