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首页> 外文期刊>Microbial Pathogenesis >The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris
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The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris

机译：在Pichia Pastoris中表达犬瘟热病毒的病毒包膜糖蛋白F的克隆

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摘要

Canine distemper virus (CDV) is a pathogen which affects members of the Canidae family, causing an acute, often fatal, systemic disease. CDV is an RNA virus of the family Paramyxoviridae that contains two envelope glycoproteins: F and HA. In this study, we focused on the envelope glycoprotein F as the main target for neutralizing antibodies produced after infection or vaccination. The complete coding region of the protein (60 kDa) was expressed in the methylotrophic yeast Pichia pastoris, obtained in a recombinant form and secreted to the culture medium. Later, to analyze its immunogenicity, the protein was combined with an oily adjuvant and used to inoculate mice. The results provide evidence supporting a potential application of this recombinant protein as a subunit vaccine.
机译：犬瘟热病毒（CDV）是一种病原体，影响Canidae家族的成员，导致急性，往往致命，全身疾病。 CDV是家庭副磷昔肽的RNA病毒，含有两个包膜糖蛋白：F和HA。 在本研究中，我们将包膜糖蛋白F重点放在感染或疫苗接种后中和抗体的主要靶标。 蛋白质（60kDa）的完整编码区域在甲基嗜型酵母Pichia牧场中表达，以重组形式获得并分泌到培养基中。 后来，分析其免疫原性，将蛋白质与油性佐剂合并并用于接种小鼠。 结果提供了支持该重组蛋白作为亚基疫苗的潜在应用的证据。

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